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Catalog Number Size Price
LS-C59222-2 2 ml (90 mg/ml) $964 
Sucrose Phosphorylase Antibody - Goat anti Sucrose Phosphorylase antibody was used to detect purified Sucrose Phosphorylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat.

Polyclonal Goat anti‑E. coli Sucrose Phosphorylase Antibody (WB) LS‑C59222

Polyclonal Goat anti‑E. coli Sucrose Phosphorylase Antibody (WB) LS‑C59222

Antibody:
Sucrose Phosphorylase Goat anti-E. coli Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified
Price
Catalog Number
$964
LS-C59222-2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Sucrose Phosphorylase Goat anti-E. coli Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified

Specifications

Description
Sucrose Phosphorylase antibody LS-C59222 is an unconjugated goat polyclonal antibody to e. coli Sucrose Phosphorylase. Validated for ELISA, IP and WB.
Target
E. coli Sucrose Phosphorylase
Host
Goat
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Sucrose Phosphorylase [E.coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Sucrose Phosphorylase [E.coli]. Cross reactivity against Sucrose Phosphorylase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Sucrose Phosphorylase [E. coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) catalog no. LS-C60340 and ABTS as a substrate for 30 minutes at room temperature.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C prior to restoration. Aliquot and store at -20°C for 12 months. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022