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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STK39 / SPAK Antibody LS‑C161567
SPAK antibody LS-C161567 is an unconjugated mouse monoclonal antibody to human SPAK (STK39). Validated for WB.
Catalog
Size
Price
LS-C161567-400
400 µl
Unavailable

Popular STK39 / SPAK Products

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-SPAK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3852 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8310 dilution 3.75 ug/ml.
Species: Human, Monkey, Rat, Bat, Dog, Hamster, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

SPAK antibody LS-C161567 is an unconjugated mouse monoclonal antibody to human SPAK (STK39). Validated for WB.
About STK39 / SPAK
STK39 / SPAK is a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. Q9UEW8 NM_013233 NP_037365.2

STK39 Antibody, Serine threonine kinase 39 Antibody, STE20/SPS1 homolog Antibody, Ste20-like protein kinase Antibody, DCHT Antibody, SPAK Antibody, Ste-20-related kinase Antibody


Specifications

Target
Human STK39 / SPAK
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Western blot (1:100 - 1:500)
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).

Western blot

STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).

Western blot

STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).

Western blot

STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
STK39 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).


Requested From: 
Date Requested: 6/25/2018

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