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IHCPlus™ STK39 / SPAK Antibody aa401‑414 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8310

Catalog Number Size Price
LS-B8310-50 50 µg (0.5 mg/ml) $425 
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-STK39 / SPAK Antibody (1µg/ml) staining of Mouse (A) and Rat (B) Brain (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
STK39 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Goat Anti-STK39 / SPAK Antibody (1µg/ml) staining of Mouse (A) and Rat (B) Brain (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
STK39 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ STK39 / SPAK Antibody aa401‑414 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8310

Note: This antibody replaces LS-C112858
Antibody:
Goat Polyclonal to Human STK39 / SPAK
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Bat, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8310-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human STK39 / SPAK
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Rat, Bat, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
SPAK antibody LS-B8310 is an unconjugated goat polyclonal antibody to SPAK (STK39 ) from human, rat, bat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human STK39 / SPAK
Host
Goat
Reactivity
Human, Monkey, Rat, Bat, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-SQEKSRRVKEENPE, from the internal region of the protein sequence according to NP_037365.2.
Blocking Peptide
LS-E27082 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8310. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa401-414
Specificity
Human STK39 / SPAK.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About STK39 / SPAK
Q9UEW8 NM_013233 NP_037365.2

Validation

Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of extracts of various cells.
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Reactivity: Human
Range: 0.781-50 ng/ml
Reactivity: All species
Range: 391-100000 pg/ml

Requested From: United States
Date Requested: 8/25/2019
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