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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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STK39 / SPAK

serine threonine kinase 39

STK39 / SPAK is a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Gene Name: serine threonine kinase 39
Family/Subfamily: Protein Kinase , FRAY
Synonyms: STK39, Serine threonine kinase 39, STE20/SPS1 homolog, Ste20-like protein kinase, DCHT, SPAK, Ste-20-related kinase
Target Sequences: NM_013233 NP_037365.2 Q9UEW8

Filtered by: None
Target
STK39 / SPAK (70)
Reactivity
Human (60)
Mouse (48)
Rat (24)
Bat (5)
Bovine (4)
Chicken (4)
Chimpanzee (3)
Dog (5)
Gibbon (3)
Guinea pig (4)
Hamster (1)
Horse (5)
Monkey (5)
Pig (4)
Rabbit (5)
Application
IHC (38)
IHC-P (11)
WB (67)
Flo (4)
ELISA (30)
IF (5)
Peptide-ELISA (9)
Host
rabbit (59)
mouse (10)
goat (1)
Isotype
IgG (47)
IgG1 (6)
IgG2b (4)
Clonality
monoclonal mc (10)
polyclonal pc (60)
Clone
OTI2E10 (2)
OTI3C12 (2)
OTI4E3 (2)
OTI4H3 (2)
Format
AP Conjugated (3)
APC (1)
APC Conjugated (4)
Azide-free (3)
Biotin Conjugated (5)
Carrier-free (4)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (4)
Unconjugated (42)
Epitope
aa346-376 (8)
aa386-415 (8)
aa433-460 (8)
aa63-545 (8)
aa151-200 (4)
250-330 aa (2)
260-340 aa (2)
aa425-450 (2)
320-400 aa, Internal (1)
Internal (1)
aa257-306 (1)
aa271-320 (1)
aa277-326 (1)
aa291-340 (1)
aa345-394 (1)
aa351-400 (1)
aa375-425 (1)
aa401-414 (1)
pSer311 (1)
pSer325 (1)
Publications
No (70)
Showing 1-25 of 70 results

IHC-plus™ STK39 / SPAK Antibody (aa401-414) LS-B8310
Goat Polyclonal to Human STK39 / SPAK
Rabbit, Dog, Rat, Hamster, Horse, Bat, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
STK39 antibody (0.5 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IHC-plus™ STK39 / SPAK Antibody LS-B3852
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $545.00
Anti-SPAK antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-SPAK antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

STK39 / SPAK Antibody (aa346-376) LS-C98726
Rabbit Polyclonal to Human STK39 / SPAK
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of SPAK (arrow) using rabbit polyclonal SPAK Antibody (A363). 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPAK gene.

STK39 / SPAK Antibody LS-C409518
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cells.

STK39 / SPAK Antibody (clone OTI4E3) LS-C114600
Mouse Monoclonal [clone OTI4E3] (IgG2b) to Human STK39 / SPAK
Flo, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

STK39 / SPAK Antibody (clone OTI4E3, Carrier-free) LS-C784323
Mouse Monoclonal [clone OTI4E3] (IgG2b) to Human STK39 / SPAK
Human
Flo, IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

STK39 / SPAK Antibody (clone OTI3C12) LS-C114601
Mouse Monoclonal [clone OTI3C12] (IgG1) to Human STK39 / SPAK
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of lung tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

STK39 / SPAK Antibody (clone OTI3C12, Carrier-free) LS-C784328
Mouse Monoclonal [clone OTI3C12] (IgG1) to Human STK39 / SPAK
Human
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of lung tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

STK39 / SPAK Antibody (clone OTI4H3) LS-C114595
Mouse Monoclonal [clone OTI4H3] (IgG2b) to Human STK39 / SPAK
Flo, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-STK39 monoclonal antibody.

STK39 / SPAK Antibody (clone OTI4H3, Carrier-free) LS-C784319
Mouse Monoclonal [clone OTI4H3] (IgG2b) to Human STK39 / SPAK
Human
Flo, IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-STK39 monoclonal antibody.

STK39 / SPAK Antibody (aa151-200) LS-C81289
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Chicken, Bovine, Rabbit, Mouse, Dog, Guinea pig, Rat, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

STK39 / SPAK Antibody (clone OTI2E10) LS-C114596
Mouse Monoclonal [clone OTI2E10] (IgG1) to Human STK39 / SPAK
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

STK39 / SPAK Antibody (clone OTI2E10, Carrier-free) LS-C784318
Mouse Monoclonal [clone OTI2E10] (IgG1) to Human STK39 / SPAK
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

STK39 / SPAK Antibody (phospho-Ser311) LS-C199616
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using STK39 (Phospho-Ser311) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from COLO205 cells, using STK39 (Phospho-Ser311) Antibody. The lane on the right is blocked with the phospho peptide.

STK39 / SPAK Antibody (aa346-376) LS-C325312
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

STK39 / SPAK Antibody (aa346-376, Biotin) LS-C270093
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IHC, WB
Biotin Conjugated
200µl  $740.00

STK39 / SPAK Antibody (aa346-376, AP) LS-C270091
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IHC, WB
AP Conjugated
200µl  $740.00

STK39 / SPAK Antibody (aa346-376, Azide-free, HRP) LS-C270094
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200µl  $740.00

STK39 / SPAK Antibody (aa346-376, PE) LS-C270098
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IHC, WB
PE Conjugated
200µl  $740.00

STK39 / SPAK Antibody (aa386-415) LS-C328709
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Human
ELISA, IHC, WB
Unconjugated
200µl  $590.00

STK39 / SPAK Antibody (250-330 aa) LS-C381399
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

STK39 / SPAK Antibody (250-330 aa) LS-C386150
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

STK39 / SPAK Antibody (320-400 aa, Internal) LS-C386152
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

STK39 / SPAK Antibody LS-C82871
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
0.1mg 1mg/ml  $365.00
Immunohistochemistry of Stk39 in human brain tissue with Stk39 antibody at 2.5 µg/mL.
Western blot of Stk39 in rat brain tissue lysate with Stk39 antibody at (A) 1 and (B) 2 ug/ml

STK39 / SPAK Antibody (aa386-415) LS-C98171
Rabbit Polyclonal to Human STK39 / SPAK
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with STK39 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
STK39 Antibody western blot of human placenta tissue lysates (35 ug/lane). The STK39 antibody detected the STK39 protein (arrow).
Showing 1-25 of 70 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).