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Anti-STK39 / SPAK Antibody (clone 4H3) LS-C114595

Catalog Size Price
LS-C114595-100 100 µl Unavailable

Related Products

74 STK39 / SPAK Antibodies

Most Popular STK39 / SPAK Antibodies

Anti-STK39 / SPAK Antibody (aa151-200) LS-C81289
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Western blot
Unconjugated
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-STK39 / SPAK Antibody (aa346-376) LS-C98726
Rabbit Polyclonal to Human STK39 / SPAK
Human, Mouse
IHC, IHC - Paraffin, Western blot
Unconjugated
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Anti-STK39 / SPAK Antibody (aa401-414) IHC-plus™ LS-B8310
Goat Polyclonal to Human STK39 / SPAK
Human, Monkey, Rat, Bat, Dog, Hamster, Horse, Rabbit
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Anti-STK39 / SPAK antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8310 dilution 3.75 ug/ml.
Anti-STK39 / SPAK Antibody (aa63-545, Biotin) LS-C689544
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Human
IHC, Western blot
Biotin Conjugated
Western blot of STK39 / SPAK antibody.
Anti-STK39 / SPAK Antibody (aa63-545, FITC) LS-C696671
Rabbit Polyclonal (IgG) to Human STK39 / SPAK
Human
IHC, Western blot
FITC Conjugated
Western blot of STK39 / SPAK antibody.

100% Guaranteed
Mouse Monoclonal [clone 4H3] (IgG2b) to Human STK39 / SPAK
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human STK39 / SPAK
Mouse
IgG2b Monoclonal [4H3]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • Western blot
  • Flow Cytometry (1:1)

Specificity and Use

STK39 / SPAK antibody was raised against protein expressed in 293T cell transfected with human STK39 expression vector
Human STK39
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About STK39 / SPAK

Q9UEW8 NM_013233 NP_037365.2

STK39 Antibody, Serine threonine kinase 39 Antibody, STE20/SPS1 homolog Antibody, Ste20-like protein kinase Antibody, DCHT Antibody, SPAK Antibody, Ste-20-related kinase Antibody

STK39 / SPAK is a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Immunohistochemistry

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-STK39 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39.

Western blot

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-STK39 monoclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-STK39 monoclonal antibody.

Requested From: 
Date Requested: 5/24/2018

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