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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SPPL2B Antibody (aa93‑142) LS‑C80685 Best Seller
SPPL2B antibody LS-C80685 is an unconjugated rabbit polyclonal antibody to SPPL2B from human and bovine. Validated for IHC and WB.
Catalog
Size
Price
LS-C80685-50
50 µg
$365
Description
SPPL2B antibody LS-C80685 is an unconjugated rabbit polyclonal antibody to SPPL2B from human and bovine. Validated for IHC and WB.
Target
Human SPPL2B
Host
Rabbit
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPPL2B antibody was raised against synthetic peptide located between aa93-142 of human SPPL2B (Q8TCT7-4, NP_001070706). Percent identity by BLAST analysis: Human, Chimpanzee (100%); Opossum (92%); Mouse, Rat, Bovine, Horse, Pig, Zebra finch, Chicken, Xenopus (85%).
Blocking Peptide
LS-E16513 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80685. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa93-142
Specificity
Human SPPL2B
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About SPPL2B
Q8TCT7 AB040965 BAA96056.1

Popular SPPL2B Products

Immunofluorescence staining of A549 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, ELISA
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Bovine
Applications: IHC, IHC - Paraffin, Western blot
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Bovine
Applications: IHC, IHC - Paraffin, Western blot
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Bovine
Applications: IHC, IHC - Paraffin, Western blot

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Images

Immunohistochemistry

SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody SPPL2B(signal peptide peptidase-like 2B) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2018
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