LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
SPPL2B Antibody (aa93‑142) LS‑C80685 Best Seller
SPPL2B antibody LS-C80685 is an unconjugated rabbit polyclonal antibody to SPPL2B from human and bovine. Validated for IHC and WB.
Catalog
Size
Price
LS-C80685-50
50 µg
Unavailable

Popular SPPL2B Products

SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Bovine
Applications: IHC, IHC - Paraffin, Western blot
SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Bovine
Applications: IHC, IHC - Paraffin, Western blot
SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence staining of A549 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Species: Human
Applications: Immunofluorescence, ELISA

Product Description

SPPL2B antibody LS-C80685 is an unconjugated rabbit polyclonal antibody to SPPL2B from human and bovine. Validated for IHC and WB.
About SPPL2B
Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. Q8TCT7 AB040965 BAA96056.1

SPPL2B Antibody, Intramembrane protease 4 Antibody, Presenilin-like protein 1 Antibody, KIAA1532 Antibody, PSH4 Antibody, PSL1 Antibody, SPP-like 2B Antibody, IMP-4 Antibody, SNPPL2B Antibody


Specifications

Target
Human SPPL2B
Host
Rabbit
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SPPL2B antibody was raised against synthetic peptide located between aa93-142 of human SPPL2B (Q8TCT7-4, NP_001070706). Percent identity by BLAST analysis: Human, Chimpanzee (100%); Opossum (92%); Mouse, Rat, Bovine, Horse, Pig, Zebra finch, Chicken, Xenopus (85%).
Blocking Peptide
LS-E16513 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80685. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa93-142
Specificity
Human SPPL2B
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SPPL2B antibody LS-C80685 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 8%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number