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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SOX3 Antibody (aa80‑129) IHC‑plus™ LS‑B1569 Best Seller
Note: This antibody replaces LS-C30036
SOX3 antibody LS-B1569 is an unconjugated rabbit polyclonal antibody to SOX3 from human, mouse, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1569-50
50 µg
$485

Popular SOX3 Products

Anti-SOX3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8332 dilution 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
MCF7 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Dog, Guinea pig, Pig, Rabbit
Applications: Western blot
SOX3 antibody LS-C109773 Western blot of PANC1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Rabbit
Applications: Western blot
Antibody
Species: Human, Rodent
Applications: IHC, IHC - Frozen, Western blot, ELISA

Product Description

SOX3 antibody LS-B1569 is an unconjugated rabbit polyclonal antibody to SOX3 from human, mouse, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About SOX3
Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. P41225 NM_005634 NP_005625.2

SOX3 Antibody, GHDX Antibody, MRGH Antibody, SOXB Antibody, Panhypopituitarism Antibody, PHPX Antibody, Sry-box3 Antibody, PHP Antibody, Transcription factor SOX-3 Antibody


Specifications

Target
Human SOX3
Host
Rabbit
Reactivity
Human, Mouse, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SOX3 antibody was raised against synthetic peptide located between aa80-129 of human SOX3 (P41225, NP_005625). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Galago, Marmoset (92%); Mouse, Rat, Dog, Rabbit (84%).
Blocking Peptide
LS-E11747 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1569. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa80-129
Specificity
Human SOX3
Usage
Immunohistochemistry: LS-B1569 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1569 was determined to be 5 ug/ml.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SOX3 antibody LS-B1569 Western blot of Raji lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SOX3 antibody LS-B1569 Western blot of Raji lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Raji Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Raji Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SOX3 antibody LS-B1569 Western blot of Raji lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SOX3 antibody LS-B1569 Western blot of Raji lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Raji Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Raji Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SOX3 antibody LS-B1569 Western blot of Raji lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SOX3 antibody LS-B1569 Western blot of Raji lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Raji Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Raji Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SOX3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1569 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SOX3 antibody LS-B1569 Western blot of Raji lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SOX3 antibody LS-B1569 Western blot of Raji lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Raji Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Raji Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/25/2018

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