Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Rabbit Monoclonal (IgG) to Human SMAD3
Human, Mouse, Rat
Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Tissue culture supernatant
Western blot (1:1000)
Flow Cytometry (1:200)
Specificity and Use
SMAD3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of Smad3.
Detects endogenous levels of total Smad3 protein. No cross reactivity is detected with other family members. Cross-Reactivity: mouse and rat. Species sequence homology: Monkey, Bovine, xenopus, and zebrafish.
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 1:100. Flow Cytometry: 1:200. Western Blot: 1:1000. Immunoprecipitation: 1:100.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.