Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- Immunofluorescence (1:100)
- Western blot (1:1000)
- Immunoprecipitation (1:100)
- Flow Cytometry (1:200)
SMAD3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of Smad3.
Detects endogenous levels of total Smad3 protein. No cross reactivity is detected with other family members. Cross-Reactivity: mouse and rat. Species sequence homology: Monkey, Bovine, xenopus, and zebrafish.
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 1:100. Flow Cytometry: 1:200. Western Blot: 1:1000. Immunoprecipitation: 1:100.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
For research use only.
This antibody carries the LSBio 100% Guarantee.