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Anti-SMAD3 Antibody (N-Terminus) LS-C39132

Catalog Size Price
LS-C39132-100 100 µl Unavailable

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146 SMAD3 Antibodies

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100% Guaranteed
Rabbit Monoclonal (IgG) to Human SMAD3
Human, Mouse, Rat
Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

Details

Human SMAD3
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Monoclonal
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Immunoprecipitation (1:100)
  • Flow Cytometry (1:200)
  • ELISA

Specificity and Use

SMAD3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues at the amino terminus of Smad3.
N-Terminus
Detects endogenous levels of total Smad3 protein. No cross reactivity is detected with other family members. Cross-Reactivity: mouse and rat. Species sequence homology: Monkey, Bovine, xenopus, and zebrafish.
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 1:100. Flow Cytometry: 1:200. Western Blot: 1:1000. Immunoprecipitation: 1:100.

Packaging

10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol, less than 0.02% sodium azide.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription.

Requested From: 
Date Requested: 10/18/2017

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