(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC (1 µg/ml)
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
SMAD3 antibody was raised against a 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3.
Recognizes human Smad3. The antibody shows very minor cross-reactivity with Smad2 (using relatively high concentrations of recombinant Smad2).
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Immunohistochemistry: 1 ug/ml Requires HIER with EDTA, pH 8.0 prior to staining. Positive control: Western Blot: human Smad3-transfected COS.
PBS, pH 7.4, 0.05% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.