SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
SMAD3 antibody was raised against a 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3.
Recognizes human Smad3. The antibody shows very minor cross-reactivity with Smad2 (using relatively high concentrations of recombinant Smad2).
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Immunohistochemistry: 1 ug/ml Requires HIER with EDTA, pH 8.0 prior to staining. Positive control: Western Blot: human Smad3-transfected COS.
PBS, pH 7.4, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.