SMAD3 antibody LS-C10221 is an unconjugated rabbit polyclonal antibody to human SMAD3. Validated for ELISA, IHC and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC (1 µg/ml)
- Western blot (1 - 3 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
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SMAD3 antibody was raised against a 20 amino acid synthetic peptide derived from a central portion of the linker domain of human Smad3.
Recognizes human Smad3. The antibody shows very minor cross-reactivity with Smad2 (using relatively high concentrations of recombinant Smad2).
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Immunohistochemistry: 1 ug/ml Requires HIER with EDTA, pH 8.0 prior to staining. Positive control: Western Blot: human Smad3-transfected COS.
PBS, pH 7.4, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.