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SMAD1 Antibody (Pro457) LS‑C177750
SMAD1 antibody LS-C177750 is an unconjugated rabbit polyclonal antibody to SMAD1 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C177750-100
100 µl
Unavailable

Popular SMAD1 Products

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
Antibody
Species: Human, Mouse, Rat
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
SMAD1 antibody LS-C80224 was used in IHC to stain formalin-fixed, paraffin-embedded human skin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila, Insect
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Immunoprecipitation, ELISA

Product Description

SMAD1 antibody LS-C177750 is an unconjugated rabbit polyclonal antibody to SMAD1 from human, mouse and rat. Validated for IHC and WB.
About SMAD1
SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. Q15797 NM_005900 NP_005891.1

SMAD1 Antibody, BSP1 Antibody, BSP-1 Antibody, JV4-1 Antibody, MADH1 Antibody, Mothers against DPP homolog 1 Antibody, JV41 Antibody, SMAD family member 1 Antibody, TGF-beta signaling protein 1 Antibody, MADR1 Antibody, HSMAD1 Antibody, MAD homolog 1 Antibody, Mad-related protein 1 Antibody, SMAD 1 Antibody


Specifications

Target
Human SMAD1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
SMAD1 antibody was raised against synthetic peptide around Pro457 of human SMAD1
Epitope
Pro457
Specificity
Human SMAD1
Presentation
PBS, pH 7.2, 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.
IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.

Western blot

Western blot of Smad1 (P457) pAb in extracts from COS7 cells.
Western blot of Smad1 (P457) pAb in extracts from COS7 cells.

Immunohistochemistry

IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.
IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.

Western blot

Western blot of Smad1 (P457) pAb in extracts from COS7 cells.
Western blot of Smad1 (P457) pAb in extracts from COS7 cells.

Immunohistochemistry

IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.
IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.

Western blot

Western blot of Smad1 (P457) pAb in extracts from COS7 cells.
Western blot of Smad1 (P457) pAb in extracts from COS7 cells.

Immunohistochemistry

IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.
IHC of Smad1 (P457) pAb in paraffin-embedded human breast carcinoma tissue.

Western blot

Western blot of Smad1 (P457) pAb in extracts from COS7 cells.
Western blot of Smad1 (P457) pAb in extracts from COS7 cells.


Requested From: 
Date Requested: 6/20/2018

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