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Antibodies > SMAD1 > LS-C331261
 
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Catalog Number Size Price
LS-C331261-20 20 µl $275 
LS-C331261-100 100 µl $389 
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded human kidney using SMAD1 antibodyat dilution of 1:100 (40x lens).
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded rat brain using SMAD1 antibodyat dilution of 1:100 (40x lens).
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded human stomach cancer tissue.
SMAD1 Antibody - Immunofluorescence analysis of HeLa cells using SMAD1 antibody. Blue: DAPI for nuclear staining.
SMAD1 Antibody - Immunofluorescence analysis of HeLa cells.
SMAD1 Antibody - Western blot analysis of extracts of various cell lines, using SMAD1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded human kidney using SMAD1 antibodyat dilution of 1:100 (40x lens).
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded rat brain using SMAD1 antibodyat dilution of 1:100 (40x lens).
SMAD1 Antibody - Immunohistochemistry of paraffin-embedded human stomach cancer tissue.
SMAD1 Antibody - Immunofluorescence analysis of HeLa cells using SMAD1 antibody. Blue: DAPI for nuclear staining.
SMAD1 Antibody - Immunofluorescence analysis of HeLa cells.
SMAD1 Antibody - Western blot analysis of extracts of various cell lines, using SMAD1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human SMAD1 Antibody (IHC, IF, WB) LS‑C331261

Polyclonal Rabbit anti‑Human SMAD1 Antibody (IHC, IF, WB) LS‑C331261

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Antibody:
SMAD1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331261-20
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SMAD1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD1 antibody LS-C331261 is an unconjugated rabbit polyclonal antibody to SMAD1 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human SMAD1
Synonyms
SMAD1 | BSP1 | BSP-1 | JV4-1 | MADH1 | Mothers against DPP homolog 1 | JV41 | SMAD family member 1 | TGF-beta signaling protein 1 | MADR1 | HSMAD1 | MAD homolog 1 | Mad-related protein 1 | SMAD 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human SMAD1 (NP_001003688.1). WKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ
Specificity
Human SMAD1
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 15kDa/52kDa, while the observed MW by Western blot was 54kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD1
Q15797 NM_005900 NP_005891.1

Publications (0)

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Customer Reviews (0)

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Featured Products

Product
SMAD1 Antibody LS‑C75853
Species: Human, Mouse, Rat
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
SMAD1 Antibody - Anti-SMAD1 Antibody - Western Blot. Western blot of Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37?C before harvest. Each lane contains approximately 15 ug of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n LS-B323) staining was used as a loading control. The membrane was washed and reacted with a 1:3000 dilution HRP-conjugated a-Rabbit IgG (p/n LS-C60865) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX.
SMAD1 Antibody LS‑C154249
Species: Human, Mouse, Rat, Dog
Applications: Western blot, Immunoprecipitation, ELISA
SMAD1 Antibody - Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
SMAD1 Antibody (clone 1D3) LS‑C133298
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
SMAD1 Antibody - SMAD1 antibody SMAD1(SMAD family member 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human skin. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (N‑Terminus) LS‑C80224
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
SMAD1 Antibody - A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SMAD1 Antibody (C‑Terminus) LS‑C31108
Species: Human
Applications: Western blot
SMAD1 Antibody - Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SMAD1 Antibody (phospho‑Ser187) LS‑C352485
Species: Human, Mouse, Rat, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
See all SMAD1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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