LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-SLC1A3 / EAAT1 Antibody LS-C149928

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SLC1A3 / EAAT1 Antibodies

Anti-SLC1A3 / EAAT1 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A9447
Rabbit Polyclonal to Human SLC1A3 / EAAT1
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Rabbit
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-SLC1A3 / EAAT1 Antibody (C-Terminus) LS-C94136
Rabbit Polyclonal (IgG) to Mouse SLC1A3 / EAAT1
Mouse, Rat
Unconjugated
Anti-SLC1A3 / EAAT1 Antibody (aa519-537) LS-C313291
Rabbit Polyclonal (IgG) to Human SLC1A3 / EAAT1
Human, Monkey, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human SLC1A3 / EAAT1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human SLC1A3 / EAAT1
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

SLC1A3 / EAAT1 antibody was raised against e. coli-derived recombinant human EAAT1/GLAST-1 His 146-Ser237 Accession # P43003.
Detects human EAAT1/GLAST-1 in IHC-P and Western blots.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SLC1A3 / EAAT1

P43003 NM_004172 NP_004163.3

SLC1A3 Antibody, EA6 Antibody, EAAT-1 Antibody, EAAT1 Antibody, GLAST Antibody, GLAST1 Antibody, GLAST-1 Antibody, GLUT-1 Antibody

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Immunohistochemistry

Immunohistochemistry
EAAT1/GLAST-1 in Human Brain. EAAT1/GLAST-1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Sheep Anti-Human EAAT1/GLAST-1 Antigen Affinity-purified Polyclonal Antibody at 3 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes and presynaptic profiles.

Western blot

Western blot
Detection of Human EAAT1/GLAST-1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 ug/ml of Sheep Anti-Human EAAT1/GLAST-1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. Specific bands were detected for EAAT1/GLAST1 at approximately 60 and 120 kDa (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/4/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number