Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SHH Antibody, HHG-1 Antibody, HLP3 Antibody, Sonic hedgehog Antibody, Sonic hedgehog homolog Antibody, MCOPCB5 Antibody, Sonic hedgehog protein Antibody, HHG1 Antibody, HPE3 Antibody, SMMCI Antibody, TPT Antibody, TPTPS Antibody
SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar.
Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 ug/ml of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kD (as indicated). This experiment was conducted under reducing conditions.
Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody. Recombinant Mouse Sonic Hedgehog N-Terminus induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N-Terminus (5 ug/ml) is neutralized (green line) by increasing concentrations of Mouse Sonic Hedgehog N-Terminus Monoclonal Antibody. The ND50 is typically 5-15 ug/ml.
Requested From: United States
Date Requested: 10/21/2016