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Anti-SHH / Sonic Hedgehog Antibody (N-Terminus, clone 171018) LS-C150256

Ordering

Wt. Vol. Conc. Price
500 µg - - $385
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SHH / Sonic Hedgehog Antibodies

Anti-SHH / Sonic Hedgehog Antibody (N-Terminus) LS-C40460
Rabbit Polyclonal (IgG) to Human SHH / Sonic Hedgehog
Human, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SHH / Sonic Hedgehog Antibody (clone 8G3) IHC-plus™ LS-B8934
Mouse Monoclonal [clone 8G3] (IgG1) to Human SHH / Sonic Hedgehog
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 171018] (IgG2a) to Mouse SHH / Sonic Hedgehog
Mouse, Human
IHC - Frozen, Western blot
Unconjugated

Details

Mouse SHH / Sonic Hedgehog
Rat
Mouse, Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [171018]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Frozen (8 - 25 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

SHH / Sonic Hedgehog antibody was raised against e. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122-Arg) Accession # Q62226.
N-Terminus
Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 1 ml
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About SHH / Sonic Hedgehog

Q15465 NM_000193 NP_000184.1

SHH Antibody, HHG-1 Antibody, HLP3 Antibody, Sonic hedgehog Antibody, Sonic hedgehog homolog Antibody, MCOPCB5 Antibody, Sonic hedgehog protein Antibody, HHG1 Antibody, HPE3 Antibody, SMMCI Antibody, TPT Antibody, TPTPS Antibody

SHH / Sonic Hedgehog is a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar.

Western blot

Western blot
Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 ug/ml of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kD (as indicated). This experiment was conducted under reducing conditions.

Neutralization

Neutralization
Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody. Recombinant Mouse Sonic Hedgehog N-Terminus induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N-Terminus (5 ug/ml) is neutralized (green line) by increasing concentrations of Mouse Sonic Hedgehog N-Terminus Monoclonal Antibody. The ND50 is typically 5-15 ug/ml.

Requested From: United States
Date Requested: 12/7/2016

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