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Catalog Number Size Price
LS-C359327-0.1 0.1 ml (1 mg/ml) $386 
SHH / Sonic Hedgehog Antibody - Immunohistochemistry: Sonic Hedgehog Antibody - IHC analysis of Sonic Hedgehog in mouse kidney.
SHH / Sonic Hedgehog Antibody - Western Blot: Sonic Hedgehog Antibody - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7
SHH / Sonic Hedgehog Antibody - Immunohistochemistry: Sonic Hedgehog Antibody - IHC analysis of Sonic Hedgehog in mouse kidney.
SHH / Sonic Hedgehog Antibody - Western Blot: Sonic Hedgehog Antibody - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7
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Polyclonal Rabbit anti‑Human SHH / Sonic Hedgehog Antibody (aa1‑75, IHC, IF, WB) LS‑C359327

Polyclonal Rabbit anti‑Human SHH / Sonic Hedgehog Antibody (aa1‑75, IHC, IF, WB) LS‑C359327

Antibody:
SHH / Sonic Hedgehog Rabbit anti-Human Polyclonal (aa1-75) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$386
LS-C359327-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SHH / Sonic Hedgehog Rabbit anti-Human Polyclonal (aa1-75) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
Sonic Hedgehog antibody LS-C359327 is an unconjugated rabbit polyclonal antibody to Sonic Hedgehog (SHH) (aa1-75) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human SHH / Sonic Hedgehog
Synonyms
SHH | HHG-1 | HLP3 | Sonic hedgehog | Sonic hedgehog homolog | MCOPCB5 | Sonic hedgehog protein | HHG1 | HPE3 | SMMCI | TPT | TPTPS
Host
Rabbit
Reactivity
Human, Mouse, Rat, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to an N-Terminus portion of the human SHH protein (between residues 1-75) [UniProt Q15465]
Epitope
aa1-75
Specificity
Human SHH / Sonic Hedgehog
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:400)
  • ICC (1:200)
  • Immunofluorescence (1:200)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This SHH antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band is observed at ~50 kDa. In Immunocytochemistry/Immunofluorescence cytoplasm/membrane staining was observed in SH-SY5Y cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SHH / Sonic Hedgehog
Q15465 NM_000193 NP_000184.1

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Requested From: United States
Date Requested: 4/25/2024