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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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SHH / Sonic Hedgehog Antibody (aa199-437) LS-C10144

Sonic Hedgehog antibody LS-C10144 is an unconjugated rat monoclonal antibody to mouse Sonic Hedgehog (SHH). Validated for ELISA and WB.
Catalog
Size
Price
LS-C10144-100
100 µg
$345
LS-C10144-500
500 µg
$770
Description
Sonic Hedgehog antibody LS-C10144 is an unconjugated rat monoclonal antibody to mouse Sonic Hedgehog (SHH). Validated for ELISA and WB.
Target
Mouse SHH / Sonic Hedgehog
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 - 2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
Immunogen
Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; aa 199-437).
Epitope
aa199-437
Specificity
Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.
Usage
Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.
Presentation
PBS, pH 7.2, 5% Trehalose
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About SHH / Sonic Hedgehog
Q15465 NM_000193 NP_000184.1

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Requested From: United States
Date Requested: 4/19/2019
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