Sonic Hedgehog antibody LS-C10144 is an unconjugated rat monoclonal antibody to mouse Sonic Hedgehog (SHH). Validated for ELISA and WB.
Mouse SHH / Sonic Hedgehog
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1 - 2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; aa 199-437).
Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not cross-react with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.
Suitable for use in Western Blot and ELISA. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1 ng/well. Western Blot: 1-2 ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5 ng/lane under non-reducing and reducing conditions.
PBS, pH 7.2, 5% Trehalose
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
About SHH / Sonic Hedgehog