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Anti-RRM2B / P53R2 Antibody (N-Terminus) LS-C154254

Note: This antibody replaces LS-C91195

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RRM2B / P53R2 Antibodies

Anti-RRM2B / P53R2 Antibody (aa2-17) IHC-plus™ LS-B545
Rabbit Polyclonal to Human RRM2B / P53R2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RRM2B / P53R2 Antibody (aa318-332) LS-C313179
Rabbit Polyclonal (IgG) to Human RRM2B / P53R2
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
Western blot
Unconjugated
Western blot Image
Anti-RRM2B / P53R2 Antibody LS-C489007
Mouse Monoclonal to Human RRM2B / P53R2
Human
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (3)
Rabbit Polyclonal (IgG) to Human RRM2B / P53R2
Human
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human RRM2B / P53R2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • IHC
  • Immunofluorescence
  • Western blot (1 µg/ml)
  • Immunoprecipitation
  • ELISA

Specificity and Use

RRM2B / P53R2 antibody was raised against anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
N-Terminus
Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan; based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.
RRM2B/p53R2 antibody is suitable for IP, IHC, IF, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kD in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (3)

1
Ribonucleotide reductase small subunit M2B prognoses better survival in colorectal cancer. Liu X, Lai L, Wang X, Xue L, Leora S, Wu J, Hu S, Zhang K, Kuo ML, Zhou L, Zhang H, Wang Y, Wang Y, Zhou B, Nelson RA, Zheng S, Zhang S, Chu P, Yen Y. Cancer research. 2011 71:3202-13. [PubMed:21415168] [PMC:PMC3085570]
2
Ribonucleotide reductase subunit p53R2 regulates mitochondria homeostasis and function in KB and PC-3 cancer cells. Wang X, Liu X, Xue L, Zhang K, Kuo ML, Hu S, Zhou B, Ann D, Zhang S, Yen Y. Biochemical and biophysical research communications. 2011 410:102-7. [PubMed:21640705] [PMC:PMC3180957]
3
RRM2B suppresses activation of the oxidative stress pathway and is up-regulated by p53 during senescence. Kuo ML, Sy AJ, Xue L, Chi M, Lee MT, Yen T, Chiang MI, Chang L, Chu P, Yen Y. Scientific reports. 2012 2:822. [PubMed:23139867] [PMC:PMC3492868]

About RRM2B / P53R2

Q7LG56 NM_015713 NP_056528.2

RRM2B Antibody, MTDPS8A Antibody, MTDPS8B Antibody, p53R2 Antibody, PEOA5 Antibody

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages.

Western blot

Western blot
Anti-RRM2B antibody - Western Blot. Western blot of affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4:293T transfected with ScRNA. Lane 5:293T transfected with p53R2 SiRNA The band at 40.7 kD, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kD. The highest band at 44.3 kD corresponds to recombinant p53R2. Primary antibody was diluted to 1 ug/mL and incubated overnight at 4C. ECL detection was used.
 

Requested From: United States
Date Requested: 12/5/2016

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