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Catalog Number Size Price
LS-B652-50 50 µl (75 mg/ml) $485 
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-pS529 shows phospho p65 staining in carcinoma cells.
RELA / NFKB p65 Antibody - Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. pan reactive anti-p65 (code# LS-B653) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the Western blot of a chemiluminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB.
RELA / NFKB p65 Antibody - Top row is anti-pan p65.  Bottom row is anti-pS529 showing phospho p65 staining in carcinoma cells.  Immunoblot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65.  Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines.  Lane 7 is a protein lysate from a primary culture of human keratinocytes.  Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and
RELA / NFKB p65 Antibody - Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells.
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
RELA / NFKB p65 Antibody - Anti-pS529 shows phospho p65 staining in carcinoma cells.
RELA / NFKB p65 Antibody - Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. pan reactive anti-p65 (code# LS-B653) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the Western blot of a chemiluminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB.
RELA / NFKB p65 Antibody - Top row is anti-pan p65.  Bottom row is anti-pS529 showing phospho p65 staining in carcinoma cells.  Immunoblot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65.  Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines.  Lane 7 is a protein lysate from a primary culture of human keratinocytes.  Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and
RELA / NFKB p65 Antibody - Anti-Human pS276 p65 Antibody - Western Blot. TNF Induces phosphorylation of p65 in KBM-5 cells.
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IHC‑plus™ Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser529, IHC, WB) LS‑B652

IHC‑plus™ Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser529, IHC, WB) LS‑B652

Note: This antibody replaces LS-C18901, LS-C9298
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer529) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B652-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer529) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-B652 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer529). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
pSer529
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS529 phosphorylated p65 from mouse, rat or other species has not been determined.
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B652 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B652 was determined to be 1:500.
Presentation
Antiserum, 0.1% sodium azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

Validation

RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LSBio Ratings

IHC-plus™ RELA / NFKB p65 Antibody (phospho-Ser529) for IHC, WB/Western, ELISA LS-B652 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)

Publications (4)

Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002 169:6490-7.

The phosphatidylinositol 3-kinase/Akt pathway negatively regulates Nod2-mediated NF-kappaB pathway. Zhao L, Lee JY, Hwang DH. Biochemical pharmacology. 2008 75:1515-25.

Role of NF-kappaB in flow-induced vascular remodeling. Castier Y, Ramkhelawon B, Riou S, Tedgui A, Lehoux S. Antioxidants & redox signaling. 2009 11:1641-9.

Anti-inflammatory and anti-catabolic effects of TENDOACTIVE on human tenocytes in vitro. Shakibaei M, Buhrmann C, Mobasheri A. Histology and histopathology. 2011 26:1173-85.

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Customer Reviews (0)


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Requested From: United States
Date Requested: 5/12/2021