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Anti-RAF1 / RAF Antibody (aa1-30) LS-C97203

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAF1 / RAF Antibodies

Anti-RAF1 / RAF Antibody (N-Terminus) IHC-plus™ LS-B6239
Mouse Monoclonal (IgG1,k) to Human RAF1 / RAF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RAF1 / RAF Antibody (clone 4G4) LS-C169472
Mouse Monoclonal [clone 4G4] (IgG1) to Human RAF1 / RAF
Human
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human RAF1 / RAF
Human
IHC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human RAF1 / RAF
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

RAF1 / RAF antibody was raised against kLH-conjugated synthetic peptide between amino acids 1-30 from N-terminal region of human RAF1.
LS-E9287 - Lyophilized - 100 µg - $145.00
aa1-30

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About RAF1 / RAF

P04049 NM_002880 NP_002871.1

RAF1 Antibody, C-Raf Antibody, C-raf-1 Antibody, CRAF Antibody, NS5 Antibody, Raf-1 Antibody, Proto-oncogene c-RAF Antibody, Oncogene RAF1 Antibody, RAF Antibody, Raf1 kinase Antibody

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of RAF1 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
Western blot of anti-RAF1 antibody in Jurkat cell lysate. RAF1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Flow Cytometry

Flow Cytometry
RAF1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/9/2016

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