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Anti-PVRL4 / Nectin 4 Antibody (aa27-351) LS-C150011

Catalog Size Price
LS-C150011-25 25 µg Unavailable
LS-C150011-100 100 µg Unavailable

Related Products

22 PVRL4 / Nectin 4 Antibodies

Most Popular PVRL4 / Nectin 4 Antibodies

Anti-PVRL4 / Nectin 4 Antibody (aa27-351) LS-C37483
Mouse Monoclonal (IgG2b) to Human PVRL4 / Nectin 4
Human
Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-PVRL4 / Nectin 4 Antibody LS-C37484
Rat Monoclonal (IgG2a) to Mouse PVRL4 / Nectin 4
Mouse
Western blot, ELISA
Unconjugated
Anti-PVRL4 / Nectin 4 Antibody (aa312-361) LS-C120225
Rabbit Polyclonal (IgG) to Human PVRL4 / Nectin 4
Human
Western blot, ELISA
Unconjugated
Western blot of extracts from NIH-3T3 cells, using PVRL4 Antibody. The lane on the right is treated with the synthesized peptide.
Anti-PVRL4 / Nectin 4 Antibody IHC-plus™ LS-B15314
Rabbit Polyclonal (IgG) to Human PVRL4 / Nectin 4
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry of Human liver cancer using PVRL4 Polyclonal Antibody at dilution of 1:60.
Anti-PVRL4 / Nectin 4 Antibody LS-C680939
Rabbit Polyclonal (IgG) to Human PVRL4 / Nectin 4
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

100% Guaranteed
Goat Polyclonal (IgG) to Human PVRL4 / Nectin 4
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Nectin-4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 ug/ml Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Details

Human PVRL4 / Nectin 4
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

Mouse myeloma cell line NS0-derived recombinant human Nectin4 Gly27-Val351 Accession # Q96NY8.
aa27-351
Detects human Nectin4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Human Nectin-4. Flow Cytometry, 2.5 µg/106 cells, MCF-7 human breast cancer cell line. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PVRL4 / Nectin 4

Q96NY8 NM_030916 NP_112178.2

PVRL4 Antibody, EDSS1 Antibody, Nectin 4 Antibody, Ig superfamily receptor LNIR Antibody, Poliovirus receptor-like 4 Antibody, LNIR Antibody, Nectin-4 Antibody, Poliovirus receptor-related 4 Antibody

PVRL4 / Nectin 4 is a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients.
(More About PVRL4 / Nectin 4)

Immunohistochemistry

Nectin-4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 ug/ml Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Nectin-4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 ug/ml Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Requested From: 
Date Requested: 4/20/2018
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