Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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order histories, retained contact details for faster checkout, review submissions, and special promotions.
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified
Applications
IHC - Paraffin (5 - 15 µg/ml)
Western blot (0.1 µg/ml)
Flow Cytometry
Specificity and Use
Mouse myeloma cell line NS0-derived recombinant human Nectin4 Gly27-Val351 Accession # Q96NY8.
aa27-351
Detects human Nectin4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Human Nectin-4. Flow Cytometry, 2.5 µg/106 cells, MCF-7 human breast cancer cell line. Immunohistochemistry, 5-15 µg/ml.
Packaging
Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
PVRL4 / Nectin 4 is a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients.
Nectin-4 in Human Placenta. Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 ug/ml Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).