Mouse PVRL4 / Nectin 4

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rmNectin-4.

Selected for its ability to detect mouse Nectin-4 in direct ELISAs and Western Blots. In these formats, this antibody shows 100% cross-reactivity with rhNectin-4, 10% cross-reactivity with rmNectin-3, and no cross-reactivity with rmNectin-1 or rmNectin-2.

• Western blot
• ELISA

Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect mouse Nectin-4. Using a colorimetric detection system, the detection limit for rmNectin-4 is approximately 20 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse Nectin-4. The detection limit for rmNectin-4 is approximately 10 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in 200ul sterile PBS.

Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96NY8 NCBI: NM_030916 NP_112178.2