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Anti-PPAT Antibody (aa51-100) LS-C80815

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPAT Antibodies

Anti-PPAT Antibody LS-C155198
Rabbit Polyclonal (IgG) to Human PPAT
Human
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-PPAT Antibody (clone 3C6) IHC-plus™ LS-B9407
Mouse Monoclonal [clone 3C6] (IgG2b) to Human PPAT
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PPAT Antibody (aa51-100, Biotin) LS-C448424
Rabbit Polyclonal (IgG) to Human PPAT
Human, Chimpanzee, Gibbon, Monkey, Bovine, Dog, Horse
IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PPAT
Human, Monkey, Bovine, Dog, Horse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PPAT
Rabbit
Human, Monkey, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

PPAT antibody was raised against synthetic peptide located between aa51-100 of human PPAT (Q06203, NP_002694). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Galago, Rabbit (92%); Elephant, Panda, Dog, Bovine, Horse, Guinea pig, Xenopus (85%); Rat (78%).
LS-E16792 - Lyophilized - 100 µg - $145.00
aa51-100
Human PPAT
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PPAT

Q06203 NM_002703 NP_002694.3

PPAT Antibody, Amidophosphoribosyltransferase Antibody, ATASE Antibody, GPAT Antibody, PRAT Antibody

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

Immunohistochemistry

Immunohistochemistry
PPAT antibody LS-C80815 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/5/2016

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