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PPAT

phosphoribosyl pyrophosphate amidotransferase

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

Gene Name: phosphoribosyl pyrophosphate amidotransferase
Synonyms: PPAT, Amidophosphoribosyltransferase, ATASE, GPAT, PRAT
Target Sequences: NM_002703 NP_002694.3 Q06203

Publications (2)

1
Quantitative analysis of purine nucleotides indicates that purinosomes increase de novo purine biosynthesis. Zhao H, Chiaro CR, Zhang L, Smith PB, Chan CY, Pedley AM, Pugh RJ, French JB, Patterson AD, Benkovic SJ. The Journal of biological chemistry. 2015 290:6705-13. (WB; Human) [Full Text Article] [PubMed:25605736] [PMC:PMC4358094]
2
Mapping Post-Translational Modifications of de Novo Purine Biosynthetic Enzymes: Implications for Pathway Regulation. Chunliang Liu, Giselle M Knudsen, Anthony M Pedley, Jingxuan He, Jared L Johnson, Tomer M Yaron, Lewis C Cantley, Stephen J Benkovic. Journal of proteome research. 2019 May;18:2078-2087. [Full Text Article] [PubMed:30964683] [PMC:PMC6499638] Related Antibodies: LS-B9407, LS-C80815.

☰ Filters
Antibodies (35)
Proteins (4)
Over-Expression Lysate (2)
Primary (35)
Recombinant (2)
PPAT (39)
Human (35)
Mouse (10)
Rat (5)
Bovine (3)
Chimpanzee (6)
Dog (5)
Gibbon (6)
Horse (3)
Monkey (11)
IHC (13)
IHC-P (8)
WB (35)
Flo (4)
ELISA (2)
ICC (1)
IF (1)
IP (1)
Peptide-ELISA (1)
rabbit (15)
mouse (20)
Product Group
IHCPlus (1)
IgG (13)
IgG1 (18)
IgG2b (2)
monoclonal mc (20)
polyclonal pc (15)
3C6 (2)
OTI1B11 (2)
OTI1B2 (2)
OTI1B8 (2)
OTI1C2 (2)
OTI1C4 (2)
OTI1C7 (2)
OTI1D9 (2)
OTI1E1 (2)
OTI2C2 (2)
Biotin Conjugated (2)
Carrier-free (10)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (29)
aa42-278 (13)
C-Terminus (4)
aa51-100 (4)
aa175-205 (1)
No (37)
Yes (2)
GST (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
PPAT Antibody - Anti-PPAT antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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PPAT Mouse anti-Human Monoclonal (3C6) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
PPAT Antibody - Immunofluorescence of methanol-fixed HeLa, using PPAT antibody at 1:500 dilution.
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PPAT Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, WB
Unconjugated
100 µl/$450
PPAT Antibody - PPAT antibody PPAT(phosphoribosyl pyrophosphate amidotransferase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PPAT Rabbit anti-Human Polyclonal (aa51-100) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$424
PPAT Antibody - PPAT antibody PPAT(phosphoribosyl pyrophosphate amidotransferase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PPAT Rabbit anti-Human Polyclonal (aa51-100) (HRP) Antibody
Dog, Bovine, Horse, Gibbon, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
PPAT Antibody - PPAT antibody PPAT(phosphoribosyl pyrophosphate amidotransferase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PPAT Rabbit anti-Human Polyclonal (aa51-100) (Biotin) Antibody
Dog, Bovine, Horse, Gibbon, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
PPAT Antibody - PPAT antibody PPAT(phosphoribosyl pyrophosphate amidotransferase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
PPAT Rabbit anti-Human Polyclonal (aa51-100) (FITC) Antibody
Dog, Bovine, Horse, Gibbon, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
PPAT Antibody - IHC of paraffin-embedded Human breast tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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PPAT Mouse anti-Human Monoclonal (aa42-278) (OTI1B11) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$434
PPAT Antibody - IHC of paraffin-embedded Human breast tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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PPAT Mouse anti-Human Monoclonal (aa42-278) (Carrier-free) (OTI1B11) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
PPAT Antibody - IHC of paraffin-embedded Human colon tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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PPAT Mouse anti-Human Monoclonal (aa42-278) (OTI1B8) Antibody
Mouse, Dog, Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100 µl/$434
PPAT Antibody - IHC of paraffin-embedded Human colon tissue using anti-PPAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
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PPAT Mouse anti-Human Monoclonal (aa42-278) (Carrier-free) (OTI1B8) Antibody
Mouse, Dog, Rat, Human, Monkey
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
PPAT Antibody - Immunohistochemistry of paraffin-embedded Human cervical cancer using PPAT Polyclonal Antibody at dilution of 1:50.
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PPAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
PPAT Antibody - Western blot analysis of extracts of HEK293 cells using PPAT antibody.
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PPAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
PPAT Antibody - Immunohistochemical analysis of Atase staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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PPAT Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
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PPAT Mouse anti-Human Monoclonal (OTI2C2) Antibody
Human
Flo, WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (aa42-278) (Carrier-free) (OTI2C2) Antibody
Human
Flo, WB
Unconjugated, Carrier-free
100 µg/$511
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (aa42-278) (OTI1B2) Antibody
Mouse, Human, Monkey
Flo, WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (aa42-278) (Carrier-free) (OTI1B2) Antibody
Mouse, Human, Monkey
Flo, WB
Unconjugated, Carrier-free
100 µg/$511
Select
PPAT Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IP, WB
Unconjugated
100 µg/$331
PPAT Antibody - Immunohistochemistry of paraffin-embedded Mouse lung tissue.
Select
PPAT Rabbit anti-Human Polyclonal Antibody
Human, Monkey
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (OTI1C7) Antibody
Human
WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (OTI1E1) Antibody
Human
WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (OTI1C4) Antibody
Human
WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (OTI1C2) Antibody
Human
WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (OTI1D9) Antibody
Human
WB
Unconjugated
100 µl/$434
PPAT Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPAT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPAT.
Select
PPAT Mouse anti-Human Monoclonal (aa42-278) (Carrier-free) (OTI1D9) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
Viewing 1-25 of 39 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).