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Anti-POLR2A / RNA polymerase II Antibody LS-C26239

Catalog Size Price
LS-C26239-100 100 µl Unavailable

Most Popular POLR2A / RNA polymerase II Antibodies

Anti-POLR2A / RNA polymerase II Antibody (phospho-Ser, clone 4H8) LS-C49196
Mouse Monoclonal [clone 4H8] to Human POLR2A / RNA polymerase II
Human, Mouse, Hamster, Drosophila, Yeast, Plant
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot Image
Anti-POLR2A / RNA polymerase II Antibody (aa1585-1634) IHC-plus™ LS-B6990
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLR2A / RNA polymerase II Antibody (C-Terminus) LS-C287522
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Xenopus, Zebrafish
Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Western blot Image
Anti-POLR2A / RNA polymerase II Antibody (aa1560-1640) LS-C385955
Rabbit Polyclonal (IgG) to Human POLR2A / RNA polymerase II
Human, Monkey, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot Image
Anti-POLR2A / RNA polymerase II Antibody (C-Terminus) IHC-plus™ LS-B14574
Rabbit Polyclonal to Human POLR2A / RNA polymerase II
Human, Monkey, Mouse, Rat, Dog, Zebrafish
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed
Mouse Monoclonal (IgG1) to Human POLR2A / RNA polymerase II
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect
IHC - Frozen, Western blot
Unconjugated

Details

Human POLR2A / RNA polymerase II
Mouse
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Tissue culture supernatant
Unmodified

Applications

  • IHC - Frozen
  • Western blot

Specificity and Use

RNA polymerase II. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
Excellent for detection of transcription activity, e. g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175000 subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200kD band of non-phosphorylated and 240kD band of phosphorylated Pol IIA. Reactivities on Cultured Cell Lines (tested so far): Drosophila chromosomes, PTK-2, HeLa, Hep2. Epitope recognized: 794DDYGPESRGFVENSYLA822. Species cross-reactivity: Human, insects.
Suitable for use in Western Blot (Western) and Immunohistochemistry (frozen tissue). Immunohistochemistry: 1:100 (recommended fixation conditions for cells: incubate in 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min). Incubation Time 1 hr at RT.

Packaging

Long term: Add glycerol (40-50%) -20°C; Short term: +4°C.
For research use only.

About POLR2A / RNA polymerase II

P24928 NM_000937 NP_000928.1

POLR2A Antibody, HRPB220 Antibody, HsRPB1 Antibody, POLR2 Antibody, RpIILS Antibody, RPOL2 Antibody, RNA Polymerase II Antibody, RPO2 Antibody, POLRA Antibody, RNA polymerase II subunit B1 Antibody, RPB1 Antibody, RPBh1 Antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other.

Requested From: 
Date Requested: 10/23/2017

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