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Anti-PDCD1 / CD279 / PD-1 Antibody (C-Terminus) IHC-plus™ LS-B2519

Note: This antibody replaces LS-C60134


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular PDCD1 / CD279 / PD-1 Antibodies

Anti-PDCD1 / CD279 / PD-1 Antibody (Internal) IHC-plus™ LS-B1387
Rabbit Polyclonal to Human PDCD1 / CD279 / PD-1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-PDCD1 / CD279 / PD-1 Antibody (clone J116) IHC-plus™ LS-B7883
Mouse Monoclonal [clone J116] (IgG1,k) to Human PDCD1 / CD279 / PD-1
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PDCD1 / CD279 / PD-1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA


Human PDCD1 / CD279 / PD-1
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA (1:10000 - 1:40000)

Specificity and Use

PDCD1 / CD279 / PD-1 antibody was raised against synthetic peptide corresponding to a region near the carboxy terminus of human PDCD1 protein.
human PDCD1 protein. This antibody reacts with PDCD1 from human, mouse and rat sources. Reactivity with PDCD1 from other sources has not been determined
Immunohistochemistry: LS-B2519 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2519 was determined to be 2.5 ug/ml. Western Blot: Expect a band at ~47 kD in size corresponding to PDCD1 in the appropriate cell lysate or extract. THP-1 cell lysate can be used as positive control.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PDCD1 / CD279 / PD-1

Q15116 NM_005018 NP_005009.2

PDCD1 Antibody, CD279 Antibody, HPD-1 Antibody, HPD-l Antibody, PD1 Antibody, Protein PD-1 Antibody, SLEB2 Antibody, PD-1 Antibody, CD279 antigen Antibody, Programmed cell death 1 Antibody

PDCD1 / CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure.


Anti-PD1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2519 concentration 2.5 ug/ml.


Anti-PD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2519 concentration 5 ug/ml.

Western blot

Western blot
Western blot using anti-PDCD1 antibody shows detection of a predominant band at ~47 kDa corresponding to PDCD1 (arrowhead) in THP-1 whole cell lysate. The predicted MW of PDCD1 is 32 kDa. PDCD1 was probed in the absence (lane A) and presence (lane B) of blocking peptide.

Requested From: United States
Date Requested: 10/24/2016

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