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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PCNA Antibody (Ready‑to‑Use) LS‑C43737
Antibody
PCNA antibody LS-C43737 is an unconjugated mouse monoclonal antibody to PCNA from human, mouse, rat and other species. Validated for IHC.
Catalog
Size
Price
LS-C43737-6000
6000 µl
Unavailable

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Product Description

PCNA antibody LS-C43737 is an unconjugated mouse monoclonal antibody to PCNA from human, mouse, rat and other species. Validated for IHC.
About PCNA
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody


Specifications

Target
Human PCNA
Host
Mouse
Reactivity
Human, Mouse, Rat, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Cynomolgus monkey, Insect, Invertebrate, Rhesus monkey, Vertebrate (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Ready-to-Use
Applications
  • IHC
  • IHC - Paraffin (1:1)
  • IHC - Frozen (1:1)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PCNA antibody was raised against rat PCNA made in the protein A expression vector pR1T2T.
Specificity
Recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kD nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with. PCNA is the auxilliary protein for DNA polymerase delta. PCNA is highly conserved between mammalian species and other vertebrates. Clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse, chicken and abalone.
Usage
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product requires antigen retrieval using heat pre-treatment prior to staining of paraffin sections.
Presentation
PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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