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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16699-50 | 50 µg (0.5 mg/ml) | $460 |
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PathPlus™ Polyclonal Goat anti‑Human PCNA Antibody (aa111‑122, IHC, WB) LS‑B16699
PathPlus™ Polyclonal Goat anti‑Human PCNA Antibody (aa111‑122, IHC, WB) LS‑B16699
Note: This antibody replaces LS-C155050
Antibody:
PCNA Goat anti-Human Polyclonal (aa111-122) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PCNA Goat anti-Human Polyclonal (aa111-122) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Specifications
Description
PCNA (Proliferating cell nuclear antigen) is a replication factor that plays critical roles in DNA replication and repair. It is a cofactor of DNA polymerase delta, and acts as a homotrimer to increase the processivity of leading strand synthesis during DNA replication. It is ubiquitinated in response to DNA damage, and involved in the RAD6-dependent DNA repair pathway. Antibodies to PCNA are used as a marker of cell proliferation. It is expressed in the nucleus of proliferating cell populations.
References: Enzymes 2016 39:231; Nature Reviews Mol Cell Biol 2014 14:269
Host
Goat
Reactivity
Human, Mouse, Rat, Pig
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EAPNQEKVSDYE, from the internal region of the protein sequence according to NP_002583.1.
Epitope
aa111-122
Specificity
Human PCNA. Reported variants represent identical protein: NP_872590.1, NP_002583.1
Applications
- IHC - Paraffin (2.5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line Jurkat (calculated MW of 28.8kD according to NP_002583.1). Recommended concentration: 0.05-0.2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Validation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ PCNA Antibody (aa111-122) for IHC, WB/Western LS-B16699 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Invertebrate, Vertebrate
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
