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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3434-0.1 | 0.1 ml (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Rat PCNA Antibody (clone PC10, IHC, WB) LS‑B3434
IHC‑plus™ Monoclonal Mouse anti‑Rat PCNA Antibody (clone PC10, IHC, WB) LS‑B3434
Note: This antibody replaces LS-C49314
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
PCNA antibody LS-B3434 is an unconjugated mouse monoclonal antibody to PCNA from rat. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB.
Host
Mouse
Reactivity
Rat, Human, Mouse, Chicken, Zebrafish, Drosophila, Yeast, Primate
(tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Clone
PC10
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein.
Specificity
Specific for PCNA p36 protein expressed at high levels in proliferating cells.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:5000)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3434 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3434 was determined to be 10 ug/ml.
Presentation
Tris-Glycine, pH 7.5, 150 mM NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Validation
Anti-PCNA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PCNA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ PCNA Antibody (clone PC10) for IHC, WB/Western, IP LS-B3434 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat, Chicken, Xenopus
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
