Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Bacillus anthracis PA / Protective Antigen
Western blot, ELISA
Rabbit Polyclonal to Bacillus anthracis PA / Protective Antigen
Bacillus anthracis PA / Protective Antigen
Bacillus anthracis (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
PA is cleaved from the 83 kD form to the 63 kD form at amino acids 164-167. Thus the (made against amino acids 186-192) should recognize both the 83 kD and 63 kD PA forms, but not the 20 kD cleavage fragment.
PBS, 0.05% BSA, 0.05% sodium azide
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF.