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Anti-NR2C1 Antibody (N-Terminus) IHC-plus™ LS-B3523

Note: This antibody replaces LS-C101685
Catalog Size Price
LS-B3523-50 50 µg Unavailable

Related Products

52 NR2C1 Antibodies

Most Popular NR2C1 Antibodies

Anti-NR2C1 Antibody (C-Terminus) LS-C30435
Rabbit Polyclonal (IgG) to Human NR2C1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NR2C1 Antibody (N-Terminus) IHC-plus™ LS-B3523
Rabbit Polyclonal (IgG) to Human NR2C1
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NR2C1 Antibody (aa515-545) LS-C323436
Rabbit Polyclonal (IgG) to Human NR2C1
Human
Western blot, ELISA
Unconjugated
Anti-NR2C1 Antibody (aa1-240) LS-C376236
Rabbit Polyclonal (IgG) to Human NR2C1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-NR2C1 Antibody LS-C412428
Rabbit Polyclonal to Human NR2C1
Human, Mouse, Rat
IHC, Western blot
Unconjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Human NR2C1
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human NR2C1
Rabbit
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bovine, Guinea pig, Hamster, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

NR2C1 antibody was raised against synthetic peptide from N-Terminus of human NR2C1 (Q15626, NP_001027458). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Pig (100%); Hamster, Bovine, Opossum, Guinea pig, Platypus, Lizard, Zebrafish (92%); Bat, Turkey, Zebra finch, Chicken, Xenopus (84%).
LS-E13754 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3523. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human NR2C1 / TR2-11
Immunohistochemistry: LS-B3523 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3523 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About NR2C1

P13056 NM_003297 NP_003288.2

NR2C1 Antibody, Orphan nuclear receptor TR2 Antibody, Testicular orphan receptor-2 Antibody, TR2 Antibody, TR2 nuclear hormone receptor Antibody, TR2-11 Antibody, Testicular receptor 2 Antibody

Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression.

Immunohistochemistry

Immunohistochemistry
Anti-NR2C1 / TR2-11 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3523 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-NR2C1 / TR2-11 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3523 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
NR2C1 / TR2-11 antibody LS-B3523 Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/23/2017

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