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Anti-NPHS1 / Nephrin Antibody (aa37-1049) LS-C150221

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular NPHS1 / Nephrin Antibodies

Anti-NPHS1 / Nephrin Antibody (C-Terminus) IHC-plus™ LS-B1382
Rabbit Polyclonal to Human NPHS1 / Nephrin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-NPHS1 / Nephrin Antibody (aa1198-1227, Biotin) LS-C264204
Rabbit Polyclonal (IgG) to Human NPHS1 / Nephrin
Human, Mouse
Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-NPHS1 / Nephrin Antibody (aa1198-1227, FITC) LS-C264206
Rabbit Polyclonal (IgG) to Human NPHS1 / Nephrin
Human, Mouse
Western blot, Flow Cytometry, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse NPHS1 / Nephrin
Mouse
IHC, Western blot
Unconjugated

Details

Mouse NPHS1 / Nephrin
Goat
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)

Specificity and Use

NPHS1 / Nephrin antibody was raised against mouse myeloma cell line NS0-derived recombinant mouse Nephrin Gln37-Thr1049 Accession # NP_062332.
aa37-1049
Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Mouse Nephrin. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
500 µl Reconstitute with sterile PBS.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About NPHS1 / Nephrin

O60500 NM_004646 NP_004637.1

NPHS1 Antibody, Nephrin Antibody, NPHN Antibody, CNF Antibody

NPHS1 / Nephrin is a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine.

Immunohistochemistry

Immunohistochemistry
Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Tissue was stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Immunohistochemistry

Immunohistochemistry
Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 ug/ml Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained (green).

Requested From: United States
Date Requested: 12/6/2016

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