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NOTCH4 Antibody (clone HMN4‑14, PE) LS‑C106197
NOTCH4 antibody LS-C106197 is a PE-conjugated hamster monoclonal antibody to mouse NOTCH4. Validated for Flow.
Catalog
Size
Price
LS-C106197-25
25 µg (0.2 mg/ml)
Unavailable
LS-C106197-100
100 µg (0.2 mg/ml)
Unavailable

Popular NOTCH4 Products

Antibody
Species: Mouse
Applications: Western blot
Anti-NOTCH4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3498 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Bovine, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry Image
Species: Human
Applications: IHC, ELISA
Anti-NOTCH4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7289 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA

Product Description

NOTCH4 antibody LS-C106197 is a PE-conjugated hamster monoclonal antibody to mouse NOTCH4. Validated for Flow.
About NOTCH4
NOTCH4 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Q99466 NM_004557 NP_004548.3

NOTCH4 Antibody, HNotch4 Antibody, Notch (Drosophila) homolog 4 Antibody, Notch homolog 4 (Drosophila) Antibody, Notch homolog 4 Antibody, INT3 Antibody, Notch 4 Antibody


Specifications

Target
Mouse NOTCH4
Host
Hamster
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [HMN4-14]
Conjugations
Phycoerythrin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
NOTCH4 antibody was raised against mouse NOTCH4
Usage
This HMN4-14 antibody has been tested by flow cytometric analysis of mouse splenocytes and Notch-4-transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Flow Cytometry

Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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