Target
Human NOTCH4
Synonyms
NOTCH4 | HNotch4 | Notch (Drosophila) homolog 4 | Notch homolog 4 (Drosophila) | Notch homolog 4 | INT3 | Notch 4
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence DHFHNGHCEKGCNN, from the internal region of the protein sequence according to NP_004548.3.
Specificity
Human NOTCH4. The immunizong peptide represents part of the extracellular domain.
Applications
- Immunofluorescence (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 27kD band in all human, mouse and rat tissue and cell line lysates tested after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 210kD according to NP_004548.3. The 27kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee