Human NOTCH4

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

E. coli-derived rhNotch-4 (aa 1778-2003).

aa1778-2003

Recognizes human Notch4.

• ICC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml to detect human Notch4 in serum starved HUVEC cells stimulated with Jagged 1. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human Notch4 intracellular domain. The detection limit for rhNotch4 intracellular domain is approximately 0.3 ng/well.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q99466 NCBI: NM_004557 NP_004548.3