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Anti-NEK6 Antibody (clone 2H7) LS-C114648

Ordering

Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular NEK6 Antibodies

Anti-NEK6 Antibody (C-Terminus) IHC-plus™ LS-A7305
Rabbit Polyclonal to Human NEK6
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-NEK6 Antibody (N-Terminus) IHC-plus™ LS-A7304
Rabbit Polyclonal to Human NEK6
Human, Monkey
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2H7] (IgG1) to Human NEK6
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human NEK6
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2H7]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:1000)
  • Immunoprecipitation

Specificity and Use

NEK6 antibody was raised against full length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.
Human NEK6
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About NEK6

Q9HC98 NM_014397 NP_055212.2

NEK6 Antibody, NIMA-related kinase 6 Antibody, Protein kinase SID6-1512 Antibody, NimA-related protein kinase 6 Antibody, SID6-1512 Antibody

NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain. The protein is believed to regulate chromatin condensation in mammalian cells via phosphorylation of histones H1 and H3, and it has been shown to phosphorylate ribosomal S6 protein kinase (S6K1) and serum and glucocorticoid induced protein kinase (SGK1), which regulate diverse cellular processes.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded endometrium tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Ovary tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6 mouse monoclonal antibody. (Dilution 1:50).

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of NEK6 by using monoclonal anti-NEK6 antibodies (Negative control: IP without adding anti-NEK6 antibody.). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-NEK6 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Requested From: United States
Date Requested: 12/4/2016

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