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Anti-mCherry Antibody LS-C204825

Catalog	Size	Price
LS-C204825-100	100 µl	Unavailable

Related Products

8 mCherry Antibodies

Most Popular mCherry Antibodies

Anti-mCherry Antibody LS-C147181

Antibody:	Rabbit Polyclonal (IgG) to Discosoma mCherry
Reactivity:	Discosoma
Application:	Western blot
Format:	Unconjugated

Anti-mCherry Antibody LS-C204207

Antibody:	Goat Polyclonal (IgG) to Discosoma mCherry
Reactivity:	Discosoma
Application:	IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-mCherry Antibody LS-C204825

Antibody:	Chicken Polyclonal (IgY) to Discosoma mCherry
Reactivity:	Discosoma
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-mCherry Antibody LS-C204826

Antibody:	Rabbit Polyclonal to Discosoma mCherry
Reactivity:	Discosoma
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Anti-mCherry Antibody (clone 1C51) LS-C204827

Antibody:	Mouse Monoclonal [clone 1C51] (IgG2a) to Discosoma mCherry
Reactivity:	Discosoma
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Antibody:	Chicken Polyclonal (IgY) to Discosoma mCherry
Reactivity:	Discosoma
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Details

Target:	Discosoma mCherry
Host:	Chicken
Clonality:	IgY Polyclonal
Conjugations:	Unconjugated
Purification:	Purified
Modifications:	Unmodified

Applications

Approved:

ICC (1:1000)
Immunofluorescence (1:1000)
Western blot (1:2000)

Specificity and Use

Immunogen:	mCherry antibody was raised against recombinant full length His tagged mCherry purified from E. coli.
Specificity:	mCherry
Usage:	Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:2000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.

Packaging

Presentation:	10 mM sodium azide.
Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Restrictions:	For research use only.

About mCherry

Description: mCherry is a fluorophore used as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria. mCherry is sometimes preferred to other fluorophores due to its color, as well as its photostability compared to other monomeric fluorophores.

(More About mCherry)

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Immunocytochemistry

HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.

Western blot

Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.

Requested From:
Date Requested: 6/24/2017

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