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mCherry Antibody LS‑C204825
MCherry antibody LS-C204825 is an unconjugated chicken polyclonal antibody to mCherry. Validated for ICC, IF and WB. Cited in 1 publication.
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Species:
Discosoma
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Species:
Discosoma
Applications:
ICC, Immunofluorescence, Western blot
Product Description
MCherry antibody LS-C204825 is an unconjugated chicken polyclonal antibody to mCherry. Validated for ICC, IF and WB. Cited in 1 publication.
About mCherry
mCherry is a fluorophore used as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria. mCherry is sometimes preferred to other fluorophores due to its color, as well as its photostability compared to other monomeric fluorophores.
Specifications
Target
Discosoma mCherry
Host
Chicken
Clonality
IgY
Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
- ICC (1:1000)
- Immunofluorescence (1:1000)
- Western blot (1:2000)
Immunogen
mCherry antibody was raised against recombinant full length His tagged mCherry purified from E. coli.
Specificity
mCherry
Usage
Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:2000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.
Presentation
10 mM sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
Publications (1)
1
Chemogenetic Activation of an Extinction Neural Circuit Reduces Cue-Induced Reinstatement of Cocaine Seeking. Augur IF, Wyckoff AR, Aston-Jones G, Kalivas PW, Peters J. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2016 36:10174-80. (IHC; Rat)
[Full Text Article]
[PubMed:27683912]
[PMC:PMC5039261]
Customer Reviews (0)
Images
Immunocytochemistry
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
Western blot
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
Immunocytochemistry
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
Western blot
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
Immunocytochemistry
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
Western blot
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
Immunocytochemistry
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
Western blot
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
Requested From:
Date Requested: 6/22/2018
Date Requested: 6/22/2018
