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Anti-mCherry Antibody LS-C204825

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular mCherry Antibodies

Anti-mCherry Antibody LS-C204207
Goat Polyclonal (IgG) to Discosoma mCherry
Discosoma
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-mCherry Antibody LS-C204825
Chicken Polyclonal (IgY) to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image
Anti-mCherry Antibody LS-C204826
Rabbit Polyclonal to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Chicken Polyclonal (IgY) to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Discosoma mCherry
Chicken
IgY Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:2000)

Specificity and Use

mCherry antibody was raised against recombinant full length His tagged mCherry purified from E. coli.
mCherry
Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:2000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.

Packaging

10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About mCherry

mCherry is a fluorophore used as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria. mCherry is sometimes preferred to other fluorophores due to its color, as well as its photostability compared to other monomeric fluorophores.

Immunocytochemistry

Immunocytochemistry
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.

Western blot

Western blot
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.

Requested From: United States
Date Requested: 12/6/2016

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