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Anti-mCherry Antibody LS-C204825

Catalog Size Price
LS-C204825-100 100 µl Unavailable

Related Products

7 mCherry Antibodies

Most Popular mCherry Antibodies

Anti-mCherry Antibody LS-C147181
Rabbit Polyclonal (IgG) to Discosoma mCherry
Discosoma
Western blot
Unconjugated
Anti-mCherry Antibody LS-C204207
Goat Polyclonal (IgG) to Discosoma mCherry
Discosoma
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot
Unconjugated
293 HEK cells transduced with Ad mCherry Rab1a and stained with anti-mCherry
Anti-mCherry Antibody LS-C204825
Chicken Polyclonal (IgY) to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
Anti-mCherry Antibody LS-C204826
Rabbit Polyclonal to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.
Anti-mCherry Antibody (clone 1C51) LS-C204827
Mouse Monoclonal [clone 1C51] (IgG2a) to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated
Shows HEK293 cells transfected with mCherry and visualized in red. The cells were stained with mCherry antibody in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and the mCherry antibody antibody. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.

100% Guaranteed Publications (1)
Chicken Polyclonal (IgY) to Discosoma mCherry
Discosoma
ICC, Immunofluorescence, Western blot
Unconjugated
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome. Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.

Details

Discosoma mCherry
Chicken
IgY Polyclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:2000)

Specificity and Use

mCherry antibody was raised against recombinant full length His tagged mCherry purified from E. coli.
mCherry
Try at dilutions of 1:1000 and higher for immunofluorescence. For western blots try at 1:2000. The mCherry protein runs at about 30kDa on SDS-PAGE gels.

Packaging

10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Chemogenetic Activation of an Extinction Neural Circuit Reduces Cue-Induced Reinstatement of Cocaine Seeking. Augur IF, Wyckoff AR, Aston-Jones G, Kalivas PW, Peters J. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2016 36:10174-80. (IHC; Rat) [Full Text Article] [PubMed:27683912] [PMC:PMC5039261]

About mCherry

mCherry is a fluorophore used as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria. mCherry is sometimes preferred to other fluorophores due to its color, as well as its photostability compared to other monomeric fluorophores.
(More About mCherry)

Immunocytochemistry

HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red. Staining with mCherry antibody is shown in Green. Green antibody staining is only seen cells which express Cherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger Cherry staining is seen in the nucleus, possibly due to degradation of some Cherry molecules to release the low molecular weight Cherry fluorochrome.

Western blot

Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
Blot of HEK293 cells transfected with pFin-EF1-mCherry vector, courtesy of the Semple-Rowland lab at the University of Florida. There is a strong clean band at about 30 kDa. HEK293 cells which were not transfected with this vector show no protein bands.
Requested From: 
Date Requested: 4/24/2018
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