LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
MCAM / CD146 Antibody (PE) LS‑C14265
CD146 antibody LS-C14265 is a PE-conjugated mouse monoclonal antibody to CD146 (MCAM) from human, mouse, dog and other species. Validated for Flow, ICC and IHC.
Antibody
Catalog
Size
Price
LS-C14265-100
100 µg (1 mg/ml)
$725

Popular MCAM / CD146 Products

Antibody
Species: Rat
Applications: Western blot, Flow Cytometry, ELISA
Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4050 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Anti-MCAM / CD146 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3628 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Angioscarcoma stained with anti-CD146
Species: Human
Applications: IHC

Product Description

CD146 antibody LS-C14265 is a PE-conjugated mouse monoclonal antibody to CD146 (MCAM) from human, mouse, dog and other species. Validated for Flow, ICC and IHC.

Specifications

Target
Human MCAM / CD146
Synonyms
MCAM, CD146, CD146 antigen, Gicerin, Melanoma adhesion molecule, MUC18, p1h12
Host
Mouse
Reactivity
Human, Mouse, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Phycoerythrin
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC (1 - 10 µg/ml)
  • Flow Cytometry (1 - 10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MCAM / CD146 antibody was raised against hUVEC.
Specificity
Recognizes human CD146 {MCAM, MUC18}. In blood and marrow, reacts only with endothelial cells. Species cross-reactivity: canine, rabbit and mouse. Does not cross-react with rat.
Usage
Suitable for use in FACS, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Flow Cytometry: 1-10 ug/ml. Immunocytochemistry: 1-10 ug/ml, EDTA- or trypsin-lifted endothelial cells. Immunohistochemistry (Frozen, fixed or unfixed): 1-10 ug/ml. For fixative, use 4% PFA for 30 minutes RT or 4?C. for 2hrs. Block with 1% BSA, 0.2% Tween-20, PBS for 30 minutes. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.6, 15 mg/ml BSA, 0.1% sodium azide.
Storage
Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing PE conjugates will result in a substantial loss of enzymatic activity.
Restrictions
For research use only.
About MCAM / CD146
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. P43121 NM_006500 NP_006491.2


Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 10/16/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy