Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B4050-50 50 µg (0.5 mg/ml) $425 
MCAM / CD146 Antibody - Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
MCAM / CD146 Antibody - Staining of HUVECs with 0.125 ug of Purified Mouse IgG1, K isotype control (blue) or 0.125 ug of Purified anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.
MCAM / CD146 Antibody - Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
MCAM / CD146 Antibody - Staining of HUVECs with 0.125 ug of Purified Mouse IgG1, K isotype control (blue) or 0.125 ug of Purified anti-human CD146 (Muc 18, MCAM, Mel-CAM, EndoCAM) (purple) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.
1 of 2
2 of 2

IHCPlus™ MCAM / CD146 Antibody Monoclonal Flo,IHC,IP,WB LS‑B4050

IHCPlus™ MCAM / CD146 Antibody Monoclonal Flo,IHC,IP,WB LS‑B4050

Note: This antibody replaces LS-C106635, LS-C14262
Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B4050-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human MCAM / CD146
Application:
IHC, IHC-P, IHC-Fr, WB, IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD146 antibody LS-B4050 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM ). Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human MCAM / CD146
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
P1H12
Conjugations
Unconjugated. Also available conjugated with FITC.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • IHC - Frozen (1 - 10 µg/ml)
  • Western blot (1 - 5 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human umbilical vein cells
Usage
This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of HUVEC cell line. This can be used at 0.25 ul per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Presentation
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

Validation

MCAM / CD146 Antibody - Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-MCAM / CD146 antibody IHC of human vessels in human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Publications (1)

Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential. Yazawa EM, Geddes-Sweeney JE, Cedeno-Laurent F, Walley KC, Barthel SR, Opperman MJ, Liang J, Lin JY, Schatton T, Laga AC, Mihm MC, Qureshi AA, Widlund HR, Murphy GF, Dimitroff CJ. The Journal of investigative dermatology. 2015 135:1849-1862. (WB; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/16/2019