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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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MCAM / CD146 Antibody (aa161‑189) LS‑C164076

Catalog Number Size Price
LS-C164076-400 400 µl $325 
Formalin-fixed and paraffin-embedded human skin reacted with MCAM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of MCAM Antibody in A2058 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified antibody.(2 ug/ml)
Flow cytometric of HeLa cells using MCAM Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skin reacted with MCAM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of MCAM Antibody in A2058 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified antibody.(2 ug/ml)
Flow cytometric of HeLa cells using MCAM Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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MCAM / CD146 Antibody (aa161‑189) LS‑C164076

Antibody:
Rabbit Polyclonal to Human MCAM / CD146
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C164076-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MCAM / CD146
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD146 antibody LS-C164076 is an unconjugated rabbit polyclonal antibody to human CD146 (MCAM ). Validated for Flow, IHC and WB.
Target
Human MCAM / CD146
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7083 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164076. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa161-189
Specificity
This MCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-189 amino acids from the Central region of human MCAM.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

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Requested From: United States
Date Requested: 8/19/2019
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