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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C164076-400 400 µl $325 
MCAM / CD146 Antibody - Formalin-fixed and paraffin-embedded human skin reacted with MCAM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MCAM / CD146 Antibody - Western blot of MCAM Antibody in A2058 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified antibody.(2 ug/ml)
MCAM / CD146 Antibody - Flow cytometric of HeLa cells using MCAM Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
MCAM / CD146 Antibody - Formalin-fixed and paraffin-embedded human skin reacted with MCAM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
MCAM / CD146 Antibody - Western blot of MCAM Antibody in A2058 cell line lysates (35 ug/lane). MCAM (arrow) was detected using the purified antibody.(2 ug/ml)
MCAM / CD146 Antibody - Flow cytometric of HeLa cells using MCAM Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human MCAM / CD146 Antibody (aa161‑189, IHC, WB) LS‑C164076

Polyclonal Rabbit anti‑Human MCAM / CD146 Antibody (aa161‑189, IHC, WB) LS‑C164076

Antibody:
Rabbit Polyclonal to Human MCAM / CD146
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C164076-400
400 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human MCAM / CD146
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD146 antibody LS-C164076 is an unconjugated rabbit polyclonal antibody to human CD146 (MCAM) (aa161-189). Validated for Flow, IHC and WB.
Target
Human MCAM / CD146
Synonyms
MCAM | CD146 | CD146 antigen | Gicerin | Melanoma adhesion molecule | MUC18 | p1h12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E7083 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-C164076. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa161-189
Specificity
This MCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-189 amino acids from the Central region of human MCAM.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCAM / CD146
P43121 NM_006500 NP_006491.2

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Requested From: United States
Date Requested: 6/6/2020