CD146 antibody LS-C14266 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM). Validated for ICC, IHC, IP and WB.
Human MCAM / CD146
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
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MCAM / CD146 antibody was raised against human primary WM902B melanoma cells.
Recognizes MEL-CAM, also known as MUC18, CD146, S-Endo-1, or A32 melanomaassociated antigen, Mr ~113kD. Species cross-reactivity: Human specific.
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-2 ug/ml detected MEL-CAM lysates from SK-Mel-37 melanoma cell lysates. SK-Mel-37 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MEL-CAM (MUC18, CD146) (1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects MEL-CAM in human melanoma samples by PAP immunohistochemistry. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate MEL-CAM from WM1366 melanoma cell lysates. Immunocytochemistry: Detects MEL-CAM in human keratinocytes.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.