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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MCAM / CD146 Antibody LS‑C14266
CD146 antibody LS-C14266 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM). Validated for ICC, IHC, IP and WB.
200 µg

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Product Description

CD146 antibody LS-C14266 is an unconjugated mouse monoclonal antibody to human CD146 (MCAM). Validated for ICC, IHC, IP and WB.
About MCAM / CD146
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. P43121 NM_006500 NP_006491.2

MCAM Antibody, CD146 Antibody, CD146 antigen Antibody, Gicerin Antibody, Melanoma adhesion molecule Antibody, MUC18 Antibody, p1h12 Antibody


Human MCAM / CD146
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein G purified
  • IHC
  • ICC
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MCAM / CD146 antibody was raised against human primary WM902B melanoma cells.
Recognizes MEL-CAM, also known as MUC18, CD146, S-Endo-1, or A32 melanomaassociated antigen, Mr ~113kD. Species cross-reactivity: Human specific.
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Western Blot: 1-2 ug/ml detected MEL-CAM lysates from SK-Mel-37 melanoma cell lysates. SK-Mel-37 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MEL-CAM (MUC18, CD146) (1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects MEL-CAM in human melanoma samples by PAP immunohistochemistry. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate MEL-CAM from WM1366 melanoma cell lysates. Immunocytochemistry: Detects MEL-CAM in human keratinocytes.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/16/2018

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