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Anti-MCAM / CD146 Antibody (Biotin) LS-C152895


Wt. Vol. Conc. Price
100 µg - 1 mg/ml Unavailable

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PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human MCAM / CD146
Human, Mouse, Dog, Rabbit
IHC - Frozen, ICC, Immunoprecipitation, Flow Cytometry, ELISA
Biotin Conjugated


Human MCAM / CD146
Human, Mouse, Dog, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Immunoaffinity purified


  • IHC - Frozen
  • ICC
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA (1 - 10 µg/ml)

Specificity and Use

MCAM / CD146 antibody was raised against immunization of mice with HUVEC
P1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs, rabbits and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375. S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphocytes are negative with or without prior stimulation. This biotinylated format is particularly useful for studies on mouse tissues when combined with streptavidin-FITC {SA103}
Immunocytochemistry: 1-10 ug/ml. Works best on EDTA or Trypsin lifted endothelial cells. Immunohistochemistry: 1-10 ug/ml. 4% PFA for 30 min RT or <2hrs @ 4°C. Block w/ 1%BSA/0.2%tween20/PBC for 30min. Works well in frozen tissue; fixed or unfixed. Immuno The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.


0.02 M PBS, 0.25 M sodium chloride, pH=7.6, 15 mg/nL BSA, 0.1% sodium azide
Store at 4°C.
For research use only.

About MCAM / CD146

P43121 NM_006500 NP_006491.2

MCAM Antibody, CD146 Antibody, CD146 antigen Antibody, Gicerin Antibody, Melanoma adhesion molecule Antibody, MUC18 Antibody, p1h12 Antibody

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development.

Requested From: 
Date Requested: 4/24/2017

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