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Anti-MBD2 Antibody (aa250-279) LS-C157879

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MBD2 Antibodies

Anti-MBD2 Antibody (aa120-151) LS-C96605
Rabbit Polyclonal to Human MBD2
Human, Mouse
Western blot
Unconjugated
Western blot Image
Anti-MBD2 Antibody (N-Terminus) LS-C109671
Rabbit Polyclonal to Human MBD2
Human, Monkey, Mouse, Rat, Bat
Western blot
Unconjugated
Western blot Image
Anti-MBD2 Antibody (Internal) IHC-plus™ LS-B7531
Rabbit Polyclonal to Human MBD2
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MBD2
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human MBD2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

MBD2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 250-279 from internal region of human MBD2.
aa250-279
Human MBD2

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About MBD2

Q9UBB5 NM_003927 NP_003918.1

MBD2 Antibody, Demethylase Antibody, DMTase Antibody, NY-CO-41 Antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with MBD2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MBD2 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
MBD2 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The MBD2 antibody detected the MBD2 protein (arrow).

Requested From: United States
Date Requested: 12/3/2016

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