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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MBD2

methyl-CpG binding domain protein 2

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.

Gene Name: methyl-CpG binding domain protein 2
Synonyms: MBD2, Demethylase, DMTase, NY-CO-41
Target Sequences: NM_003927 NP_003918.1 Q9UBB5

Filtered by: None
Target
MBD2 (51)
Reactivity
Human (51)
Mouse (26)
Rat (16)
Bat (9)
Bovine (5)
Chicken (4)
Chimpanzee (6)
Dog (2)
Gibbon (3)
Guinea pig (4)
Hamster (1)
Horse (5)
Monkey (10)
Orangutan (3)
Pig (6)
Rabbit (5)
Application
IHC (5)
IHC-P (4)
WB (50)
ELISA (20)
IF (1)
IP (2)
Peptide-ELISA (1)
Host
rabbit (48)
mouse (1)
goat (1)
chicken (1)
Isotype
IgG (29)
IgG1,k (1)
Clonality
monoclonal mc (1)
polyclonal pc (50)
Clone
1812CT625.9.45 (1)
Format
AP Conjugated (2)
APC (1)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (5)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (3)
Unconjugated (26)
Epitope
aa120-151 (8)
aa145-411 (8)
aa250-279 (8)
N-Terminus (4)
aa151-200 (4)
Internal (2)
aa11-25 (1)
aa15-29 (1)
aa159-411 (1)
aa187-200 (1)
aa361-411 (1)
Publications
No (51)
Showing 1-25 of 51 results

IHC-plus™ MBD2 Antibody (aa15-29) LS-B3603
Goat Polyclonal to Human MBD2
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-MBD2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-MBD2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

IHC-plus™ MBD2 Antibody (Internal) LS-B7531
Rabbit Polyclonal to Human MBD2
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $425.00
Anti-MBD2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

MBD2 Antibody LS-C136964
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl  $275.00
Western blot Image

IHC-plus™ MBD2 Antibody (aa361-411) LS-B12336
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl 0.2mg/ml  $425.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection of Human MBD2 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1 Detection: DAB.

MBD2 Antibody (aa250-279) LS-C157879
Rabbit Polyclonal to Human MBD2
Human
IF, WB
Unconjugated
400µl  $325.00
Fluorescent confocal image of HeLa cell stained with MBD2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MBD2 immunoreactivity is localized to Nucleus significantly.
MBD2 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The MBD2 antibody detected the MBD2 protein (arrow).

MBD2 Antibody (aa151-200) LS-C30730
Rabbit Polyclonal (IgG) to Human MBD2
Chicken, Bovine, Rat, Pig, Horse, Bat, Rabbit, Mouse, Guinea pig, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $325.00
A: Marker, B: HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

MBD2 Antibody (N-Terminus) LS-C109671
Rabbit Polyclonal to Human MBD2
Mouse, Rat, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
MBD2 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

MBD2 Antibody (aa120-151) LS-C96605
Rabbit Polyclonal to Human MBD2
Mouse, Human
WB
Unconjugated
400µl  $325.00
The anti-MBD2 N-term Antibody is used in Western blot to detect MBD2 in A375 cell lysate (lane 1) and mouse brain tissue lysate (lane 2) lysate.

MBD2 Antibody (Internal) LS-C153492
Rabbit Polyclonal to Human MBD2
Human
ELISA, IHC, IP, WB
Unconjugated
100µg 100µl 1mg/ml  $315.00

MBD2 Antibody (clone 1812CT625.9.45) LS-C757804
Mouse Monoclonal [clone 1812CT625.9.45] (IgG1,k) to Human MBD2
Human
ELISA, IHC-P, WB
Unconjugated
200µl  $325.00
MBD2 Antibody staining MBD2 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes: Anti-MBD2 Antibody at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

MBD2 Antibody LS-C768071
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Rat, Human
IHC, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

MBD2 Antibody LS-C807030
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

MBD2 Antibody (aa250-279) LS-C322877
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
Unconjugated
200µl  $590.00

MBD2 Antibody (aa250-279, Biotin) LS-C246486
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

MBD2 Antibody (aa250-279, AP) LS-C237420
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
AP Conjugated
200µl  $740.00

MBD2 Antibody (aa250-279, APC) LS-C237421
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
APC Conjugated
200µl  $740.00

MBD2 Antibody (aa250-279, PE) LS-C237422
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
PE Conjugated
200µl  $740.00

MBD2 Antibody (aa250-279, Azide-free, HRP) LS-C248771
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

MBD2 Antibody (aa120-151) LS-C322881
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
ELISA, WB
Unconjugated
200µl  $590.00

MBD2 Antibody (aa120-151, Biotin) LS-C237425
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

MBD2 Antibody (aa120-151, FITC) LS-C237427
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
ELISA, WB
FITC Conjugated
200µl  $740.00

MBD2 Antibody (aa120-151, APC) LS-C237424
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
ELISA, WB
APC Conjugated
200µl  $740.00

MBD2 Antibody (aa120-151, PE) LS-C237429
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
ELISA, WB
PE Conjugated
200µl  $740.00

MBD2 Antibody (aa120-151, Azide-free, HRP) LS-C248773
Rabbit Polyclonal (IgG) to Human MBD2
Mouse, Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

MBD2 Antibody LS-C666666
Rabbit Polyclonal (IgG) to Human MBD2
Human
ELISA, WB
Unconjugated
100µg  $335.00
Showing 1-25 of 51 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).