Target
Human MAP2K2 / MKK2 / MEK2
Synonyms
MAP2K2 | ERK activator kinase 2 | MAP kinase kinase 2 | MAPK/ERK kinase 2 | MAPKK 2 | MKK2 | MEK 2 | MEK2 | MAPKK2 | PRKMK2
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DLKMLTNHTFIKRS, from the internal region of the protein sequence according to NP_109587.1.
Specificity
Human MAP2K2 / MKK2 / MEK2
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kD band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kD according to NP_109587.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP2K2 / MKK2 / MEK2