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Anti-MAP2K2 / MKK2 / MEK2 Antibody (aa359-372) LS-C205037

Catalog Size Price
LS-C205037-100 100 µg (0.5 mg/ml) Unavailable

Most Popular MAP2K2 / MKK2 / MEK2 Antibodies

Anti-MAP2K2 / MKK2 / MEK2 Antibody (phospho-Thr394) IHC-plus™ LS-B1727
Rabbit Polyclonal (IgG) to Human MAP2K2 / MKK2 / MEK2
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Anti-MAP2K2 / MEK2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1727 concentration 10 ug/ml.
Anti-MAP2K2 / MKK2 / MEK2 Antibody (N-Terminus) IHC-plus™ LS-B4671
Rabbit Polyclonal (IgG) to Human MAP2K2 / MKK2 / MEK2
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-MAP2K2 / MEK2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4671 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-MAP2K2 / MKK2 / MEK2 Antibody (clone 1A2) LS-C337945
Mouse Monoclonal [clone 1A2] (IgG2a) to Human MAP2K2 / MKK2 / MEK2
Human, Monkey, Mouse, Rat, Dog
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
IHC of paraffin-embedded Human liver tissue using anti-MAP2K2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Anti-MAP2K2 / MKK2 / MEK2 Antibody LS-C497776
Rabbit Monoclonal to Human MAP2K2 / MKK2 / MEK2
Human
IHC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-MAP2K2 / MKK2 / MEK2 Antibody LS-C500111
Rabbit Polyclonal (IgG) to Human MAP2K2 / MKK2 / MEK2
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA04909A0Rb at dilution of 1:100

100% Guaranteed
Goat Polyclonal to Human MAP2K2 / MKK2 / MEK2
Human, Monkey
Western blot, ELISA
Unconjugated

Details

Human MAP2K2 / MKK2 / MEK2
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:128000)

Specificity and Use

Synthetic peptide C-DLKMLTNHTFIKRS from an internal region of human MAP2K2 / MKK2 / MEK2 (NP_109587.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Bovine, Elephant, Pig, Turkey, Chicken (93%); Rat, Ferret, Bat, Panda, Horse, Guinea pig, Zebra finch, Trout, Medaka, Catfish, Salmon, Zebrafish (86%); Mouse (79%).
aa359-372
Human MAP2K2 / MKK2 / MEK2
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kD band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kD according to NP_109587.1). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About MAP2K2 / MKK2 / MEK2

P36507 NM_030662 NP_109587.1

MAP2K2 Antibody, ERK activator kinase 2 Antibody, MAP kinase kinase 2 Antibody, MAPK/ERK kinase 2 Antibody, MAPKK 2 Antibody, MKK2 Antibody, MEK 2 Antibody, MEK2 Antibody, MAPKK2 Antibody, PRKMK2 Antibody

MAP2K2 / MKK2 / MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases.

Western blot

MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/22/2018

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