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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP2K2 / MKK2 / MEK2 Antibody (aa359‑372) LS‑C205037
MEK2 antibody LS-C205037 is an unconjugated goat polyclonal antibody to MEK2 (MAP2K2 / MKK2) from human and monkey. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C205037-100
100 µg (0.5 mg/ml)
Unavailable

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Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

MEK2 antibody LS-C205037 is an unconjugated goat polyclonal antibody to MEK2 (MAP2K2 / MKK2) from human and monkey. Validated for Peptide-ELISA and WB.
About MAP2K2 / MKK2 / MEK2
MAP2K2 / MKK2 / MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. P36507 NM_030662 NP_109587.1

MAP2K2 Antibody, ERK activator kinase 2 Antibody, MAP kinase kinase 2 Antibody, MAPK/ERK kinase 2 Antibody, MAPKK 2 Antibody, MKK2 Antibody, MEK 2 Antibody, MEK2 Antibody, MAPKK2 Antibody, PRKMK2 Antibody


Specifications

Target
Human MAP2K2 / MKK2 / MEK2
Host
Goat
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Bovine, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Immunogen
Synthetic peptide C-DLKMLTNHTFIKRS from an internal region of human MAP2K2 / MKK2 / MEK2 (NP_109587.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Bovine, Elephant, Pig, Turkey, Chicken (93%); Rat, Ferret, Bat, Panda, Horse, Guinea pig, Zebra finch, Trout, Medaka, Catfish, Salmon, Zebrafish (86%); Mouse (79%).
Epitope
aa359-372
Specificity
Human MAP2K2 / MKK2 / MEK2
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kD band observed in lysates of cell lines Daudi, Jurkat, K562 and Molt4 (calculated MW of 44.4kD according to NP_109587.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
MAP2K2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B), K562 (C) and Molt4 (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/20/2018

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