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Catalog Number Size Price
LS-C357647-120 120 Tests $536 
LRRC32 Antibody - Flow cytometry of LRRC32 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human LRRC32 Antibody (clone ANC8C9, FITC) LS‑C357647

Monoclonal Mouse anti‑Human LRRC32 Antibody (clone ANC8C9, FITC) LS‑C357647

Antibody:
LRRC32 Mouse anti-Human Monoclonal (FITC) (ANC8C9) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$536
LS-C357647-120
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
LRRC32 Mouse anti-Human Monoclonal (FITC) (ANC8C9) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
FITC, Unmodified
Other formats:

Specifications

Description
LRRC32 antibody LS-C357647 is an FITC-conjugated mouse monoclonal antibody to human LRRC32. Validated for ELISA and Flow.
Target
Human LRRC32
Synonyms
LRRC32 | D11S833E | GARP | Garpin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
ANC8C9
Conjugations
FITC. Also available Unconjugated or conjugated with RPE, Biotin.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human GARP.
Specificity
Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
Applications
  • Flow Cytometry
  • ELISA
  • Applications tested for the base form of this product only
Usage
: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate, and anti-human GARP/FITC at dilution factor of 1:50 (5 ug/ml). Cells were washed twice, fixed, permeabolized and co stained with anti-FOXP3/PE after which they were washed twice and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 2.1% sub population of FOXP3 positive cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/FITC isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LRRC32
Q14392 NM_005512 NP_005503.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024