Target
Human LRRC32
Synonyms
LRRC32 | D11S833E | GARP | Garpin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Conjugations
FITC.
Also available
Unconjugated or conjugated with
RPE,
Biotin.
Purification
Protein A purified
Immunogen
Recombinant human GARP.
Specificity
Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
Applications
- Flow Cytometry
- ELISA
- Applications tested for the base form of this product only
Usage
: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate, and anti-human GARP/FITC at dilution factor of 1:50 (5 ug/ml). Cells were washed twice, fixed, permeabolized and co stained with anti-FOXP3/PE after which they were washed twice and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 2.1% sub population of FOXP3 positive cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/FITC isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee