Human LRRC32

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

ANC8C9

R. Phycoerythrin. Also available Unconjugated or conjugated with FITC, Biotin.

Protein A purified

Unmodified

Recombinant human GARP.

Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.

• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

(FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate, and anti-human GARP/PE at dilution factor of 1:50 (10 ug/ml). Cells were washed twice, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 3.9% sub population of gated cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG2a/PE isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA

Store at 4°C. Do not freeze. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14392 NCBI: NM_005512 NP_005503.1