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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LRRC32 Antibody (clone ANC8C9) LS‑C357662
LRRC32 antibody LS-C357662 is an unconjugated mouse monoclonal antibody to human LRRC32. Validated for ELISA and Flow.
Catalog
Size
Price
LS-C357662-100
100 µg (1 mg/ml)
$345
Description
LRRC32 antibody LS-C357662 is an unconjugated mouse monoclonal antibody to human LRRC32. Validated for ELISA and Flow.
Target
Human LRRC32
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [ANC8C9]
Conjugations
Unconjugated. Also available conjugated with FITC, RPE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
LRRC32 antibody was raised against recombinant human GARP.
Specificity
Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
Usage
(FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About LRRC32
Q14392 NM_005512 NP_005503.1

Popular LRRC32 Products

Formalin-fixed and paraffin-embedded human cerebellum reacted with GARP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot of GARP/LRRC32 in mouse thymus lysate in the 1) absence and 2) presence of immunizing peptide using Polyclonal Antibody to GARP/LRRC32 at 0.5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Species: Human, Monkey, Mouse, Rat
Applications: Western blot, Flow Cytometry
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Western blot of recombinant GARP / LRRC32.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot

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Flow Cytometry

Flow cytometry of LRRC32 antibody
Flow cytometry of LRRC32 antibody

Flow Cytometry

Flow cytometry of LRRC32 antibody
Flow cytometry of LRRC32 antibody

Flow Cytometry

Flow cytometry of LRRC32 antibody
Flow cytometry of LRRC32 antibody

Flow Cytometry

Flow cytometry of LRRC32 antibody
Flow cytometry of LRRC32 antibody

Requested From: United States
Date Requested: 11/19/2018
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