LRRC32 antibody LS-C357662 is an unconjugated mouse monoclonal antibody to human LRRC32. Validated for ELISA and Flow.
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
Protein A purified
- Flow Cytometry
- (applications tested for the base form of this product only)
LRRC32 antibody was raised against recombinant human GARP.
Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
(FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb.
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin
Store at 4°C, avoid freeze-thaw cycles.
For research use only.