Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4C4] (IgG1) to Human LMNA / Lamin A+C
Human, Mouse, Rat, Bovine, Pig
ICC, Immunofluorescence, Western blot
Human LMNA / Lamin A+C
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4C4]
Western blot (1:10000)
Specificity and Use
LMNA / Lamin A+C antibody was raised against full length recombinant human Lamin C.
Clone 4C4 is known to react with Lamin A/C from human, cow, pig, mouse, rat and other mammals. Since Lamin A/C is highly conserved in protein sequence, it is likely that the antibody is effective on other species also.
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
PBS, 10 mM sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B.
HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with chicken polyclonal antibody to Vimentin Vim (green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.
Stripe blots of crude HeLa cell extract stained with MCS-4C4. Note two strong clean bands at 74 kDa and 64 kDa, corresponding to Lamin A and C, in the lane labeled 6. Lane 5 was stained with our antibody to Enolase 1, which we will market shortly. Lanes 3 and 4 were reacted with 5J11, a monoclonal antibody to all six actin isotypes. Molecular weights of each protein are as indicated.
Requested From: United States
Date Requested: 1/23/2017