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Anti-LCAT Antibody (aa362-411) LS-C80450

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - Unavailable

Most Popular LCAT Antibodies

Anti-LCAT Antibody (C-Terminus) IHC-plus™ LS-B252
Rabbit Polyclonal to Human LCAT
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-LCAT Antibody (aa285-313) LS-C165713
Rabbit Polyclonal to Human LCAT
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-LCAT Antibody (clone 4A9) LS-C197605
Mouse Monoclonal [clone 4A9] (IgG2a,k) to Human LCAT
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human LCAT
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human LCAT
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

LCAT antibody was raised against synthetic peptide located between aa362-411 of human LCAT (P04180, NP_000220). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Galago, Bat, Opossum (92%); Platypus (91%); Rabbit (85%); Catfish (83%).
aa362-411
Human LCAT
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About LCAT

P04180 NM_000229 NP_000220.1

LCAT Antibody

Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines.

Western blot

Western blot
Transfected 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/27/2017

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