Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Reacts with human cytokeratin 10 found in suprabasal cells of stratified squamous epithelia.
Suitable for use in Immunoperoxidase (frozen sections), Western Blot, and Immunohistochemistry (formalin fixed sections). Immunohistochemistry: 1:20-1:100 (1-5 ug/ml). Pretreatment of formalin fixed sections with protease type XXIV (concentration 500 ug/ml) produces best results.
PBS, 10 mM sodium azide, 0.1% BSA.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
KRT10 / CK10 / Cytokeratin 10 encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis.