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Anti-KLF4 Antibody (aa80-129) IHC-plus™ LS-B1192

Note: This antibody replaces LS-C31125

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular KLF4 Antibodies

Anti-KLF4 Antibody (aa1-170, clone AT4E6) IHC-plus™ LS-B2966
Mouse Monoclonal [clone AT4E6] (IgG1,k) to Human KLF4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-KLF4 Antibody (aa401-450) IHC-plus™ LS-B5641
Rabbit Polyclonal (IgG) to Human KLF4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KLF4 Antibody (aa2-470) LS-C149986
Goat Polyclonal (IgG) to Human KLF4
Human
IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human KLF4
Human, Monkey, Bovine, Dog, Horse, Pig, Sheep
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human KLF4
Rabbit
Human, Monkey, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (2.5 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

KLF4 antibody was raised against synthetic peptide located between aa80-129 of human KLF4 (O43474, NP_004226). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%); Galago, Dog, Bat, Horse (86%); Sheep, Elephant, Panda, Cat, Bovine, Pig (80%).
LS-E12695 - Lyophilized - 100 µg - $145.00
aa80-129
Human KLF4
Immunohistochemistry: LS-B1192 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1192 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About KLF4

O43474 NM_004235 NP_004226.3

KLF4 Antibody, EZF Antibody, GKLF Antibody, Kruppel-like factor 4 (gut) Antibody, Hezf Antibody, Krueppel-like factor 4 Antibody

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface.

Immunohistochemistry

Immunohistochemistry
Anti-KLF4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1192 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
KLF4 antibody LS-B1192 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Transfected 293T Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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